- 7C9 (See other available formats)
- Regulatory Status
- Other Names
- Sialic acid-binding Ig-like lectin 8 (Siglec-8), Siglec8L, Sialoadhesin family member 2 (SAF2)
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
Siglec-8 is a lectin specific for 6'-sulfo-sLex and a member of the Ig-superfamily. It is expressed almost exclusively in eosinophils; however, basophils and mast cells can express it to a lower degree. Siglec-8 is a 54 kD transmembranal protein; the extracellular domain has one V-set Ig-like domain and two C2-set domains. The cytoplasmic domain has two immunoreceptor tyrosine-based inhibitor motifs (ITIM) that recruit SH2-family phosphatases after tyrosine phosphorylation. There are reports that siglec-8 inhibits the release of histamine and prostaglandin D2 mediated by the IgEFcR. This molelcule is also involved in the induction of apoptosis.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Recombinant Siglec-8 fused to human IgG Fc
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
(PubMed link indicates BioLegend citation)
- Floyd H, et al. 2000. J. Biol. Chem. 275:861.
- Wen T, et al. 2014. J Immunol. 192:5481. PubMed
- Product Citations
AB_2629715 (BioLegend Cat. No. 347107)
AB_2629716 (BioLegend Cat. No. 347108)
- A lectin of 54 kD, belonging to the Ig-superfamily; has one V-set Ig-like domain and two C2-set domains; has a single transmembrane domain, and two immunoreceptor tyrosine-based inhibitor motifs (ITIM) in the cytoplasmic domain
Eosinophils, basophils, mast cells
- Cell adhesion, signal transduction
- Phosphatases containing a SH2 domain
- Inhibition of IgE receptor-triggered histamine and prostaglandin D2 release, apoptosis
- Cell Type
- Basophils, Eosinophils, Mast cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Adhesion Molecules, Siglec Molecules
- Antigen References
1. Bochner BS, et al. 2009. Clin. Exp. Allergy. 39:317.
2. Hudson SA, et al. 2009. J. Pharmacol. Exp. Ther. 330:608.
3. Nutku E, et al. 2005. Biochem. Biophys. Res. Commun. 336:918.
- Gene ID
- 27181 View all products for this Gene ID
- View information about Siglec-8 on UniProt.org
Related Pages & Pathways
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other FormatsView All Siglec-8 Reagents Request Custom Conjugation
|Purified anti-human Siglec-8||7C9||FC|
|PE anti-human Siglec-8||7C9||FC|
|APC anti-human Siglec-8||7C9||FC|
|PE/Dazzle™ 594 anti-human Siglec-8||7C9||FC|
|PE/Cyanine7 anti-human Siglec-8||7C9||FC|
|PerCP/Cyanine5.5 anti-human Siglec-8||7C9||FC|
|TotalSeq™-C0199 anti-human Siglec-8||7C9||PG|
|PE/Cyanine5 anti-human Siglec-8||7C9||FC|
|TotalSeq™-D0199 anti-human Siglec-8||7C9||PG|
|Biotin anti-human Siglec-8||7C9||FC|
|Alexa Fluor® 647 anti-human Siglec-8||7C9||FC|
|TotalSeq™-A0199 anti-human Siglec-8||7C9||PG|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human Siglec-8
PE anti-human Siglec-8
APC anti-human Siglec-8
PE/Dazzle™ 594 anti-human Siglec-8
PE/Cyanine7 anti-human Siglec-8
PerCP/Cyanine5.5 anti-human Siglec-8
TotalSeq™-C0199 anti-human Siglec-8
PE/Cyanine5 anti-human Siglec-8
TotalSeq™-D0199 anti-human Siglec-8
Biotin anti-human Siglec-8
Alexa Fluor® 647 anti-human Siglec-8
TotalSeq™-A0199 anti-human Siglec-8