Loading...

PerCP/Cyanine5.5 anti-human Siglec-8 Antibody

Pricing & Availability
Clone
7C9 (See other available formats)
Regulatory Status
RUO
Other Names
Sialic acid-binding Ig-like lectin 8 (Siglec-8), Siglec8L, Sialoadhesin family member 2 (SAF2)
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
7C9_PerCPCyanine55_Siglec8_Antibody_102318
Human peripheral blood leukocytes were stained with CD193 (CCR3) APC and Siglec-8 (clone 7C9) PerCP/Cyanine5.5 (left), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right). Data shown was gated on the granulocyte cell population.
  • 7C9_PerCPCyanine55_Siglec8_Antibody_102318
    Human peripheral blood leukocytes were stained with CD193 (CCR3) APC and Siglec-8 (clone 7C9) PerCP/Cyanine5.5 (left), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right). Data shown was gated on the granulocyte cell population.
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Save
347107 25 tests ¥34,650
347108 100 tests ¥75,180
Description

Siglec-8 is a lectin specific for 6'-sulfo-sLex and a member of the Ig-superfamily. It is expressed almost exclusively in eosinophils; however, basophils and mast cells can express it to a lower degree. Siglec-8 is a 54 kD transmembranal protein; the extracellular domain has one V-set Ig-like domain and two C2-set domains. The cytoplasmic domain has two immunoreceptor tyrosine-based inhibitor motifs (ITIM) that recruit SH2-family phosphatases after tyrosine phosphorylation. There are reports that siglec-8 inhibits the release of histamine and prostaglandin D2 mediated by the IgEFcR. This molelcule is also involved in the induction of apoptosis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Siglec-8 fused to human IgG Fc
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Floyd H, et al. 2000. J. Biol. Chem. 275:861.
  2. Wen T, et al. 2014. J Immunol. 192:5481. PubMed
Product Citations
  1. Vijayakumar B, et al. 2022. Immunity. . PubMed
  2. Rohner L, et al. 2020. Sci Rep. 0.570833333. PubMed
  3. Rodrigues E, et al. 2020. Nat Commun. 3.99375. PubMed
RRID
AB_2629715 (BioLegend Cat. No. 347107)
AB_2629716 (BioLegend Cat. No. 347108)

Antigen Details

Structure
A lectin of 54 kD, belonging to the Ig-superfamily; has one V-set Ig-like domain and two C2-set domains; has a single transmembrane domain, and two immunoreceptor tyrosine-based inhibitor motifs (ITIM) in the cytoplasmic domain
Distribution

Eosinophils, basophils, mast cells

Function
Cell adhesion, signal transduction
Interaction
Phosphatases containing a SH2 domain
Bioactivity
Inhibition of IgE receptor-triggered histamine and prostaglandin D2 release, apoptosis
Cell Type
Basophils, Eosinophils, Mast cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family
Adhesion Molecules, Siglec Molecules
Antigen References

1. Bochner BS, et al. 2009. Clin. Exp. Allergy. 39:317.
2. Hudson SA, et al. 2009. J. Pharmacol. Exp. Ther. 330:608.
3. Nutku E, et al. 2005. Biochem. Biophys. Res. Commun. 336:918.

Gene ID
27181 View all products for this Gene ID
UniProt
View information about Siglec-8 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 10/23/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human Siglec-8

  • PE anti-human Siglec-8

  • APC anti-human Siglec-8

  • PE/Dazzle™ 594 anti-human Siglec-8

  • PE/Cyanine7 anti-human Siglec-8

  • PerCP/Cyanine5.5 anti-human Siglec-8

  • TotalSeq™-C0199 anti-human Siglec-8

  • PE/Cyanine5 anti-human Siglec-8

  • TotalSeq™-D0199 anti-human Siglec-8

  • Biotin anti-human Siglec-8

  • Alexa Fluor® 647 anti-human Siglec-8

  • TotalSeq™-A0199 anti-human Siglec-8

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account