PerCP/Cyanine5.5 anti-human IgE Antibody

Pricing & Availability
MHE-18 (See other available formats)
Regulatory Status
Other Names
Immunoglobulin E
Mouse IgG1, κ
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Product Citations
Human peripheral blood leukocytes were stained with CCR3 APC and anti-human IgE (clone MHE-18) PerCP/Cyanine5.5 (top) or mouse IgG1, ? PerCP/Cyanine5.5 (bottom). Data shown was gated on lymphocyte population.
  • MHE-18_PerCPCy5.5_1_070212
    Human peripheral blood leukocytes were stained with CCR3 APC and anti-human IgE (clone MHE-18) PerCP/Cyanine5.5 (top) or mouse IgG1, ? PerCP/Cyanine5.5 (bottom). Data shown was gated on lymphocyte population.
  • MHE-18_PerCPCy5.5_2_070212
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Save
325511 25 tests ¥300.00
325512 100 tests ¥650.00

IgE is the seventh immunoglobulin made by B cells in the immune response.

Product Details
Technical data sheet

Product Details

Antibody Type
Host Species
Human Ig cocktail
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The MHE-18 antibody reacts with both soluble and membrane human immunoglobulin E (IgE). It does not react with other Ig isotypes. Some formats of the MHE-18 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Clark D, et al. 2013. J Immunol Methods. 22:1759. PubMed.
Product Citations
  1. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
AB_11218987 (BioLegend Cat. No. 325511)
AB_11219590 (BioLegend Cat. No. 325512)

Antigen Details


B cells

Cell Type
B cells
Biology Area
Gene ID
3497 View all products for this Gene ID
View information about IgE on

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 05/24/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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