- LN3 (See other available formats)
- Regulatory Status
- Other Names
- Major Histocompatibility Class II, MHC class II
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
The LN3 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.Product Details
- Verified Reactivity
- Reported Reactivity
- Antibody Type
- Host Species
- human PBL
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining1 of frozen sections and formalin-fixed paraffin-embedded sections1, and immunoprecipitation1.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
(PubMed link indicates BioLegend citation)
- Marder RJ, et al. 1985. Lab. Invest. 52:497.
- Norton AJ and Isaacson PG. 1987. Am. J. Pathol. 128:225.
- Hua ZX, et al. 1998. Hum. Pathol. 29(12):1441.
- Product Citations
AB_2721717 (BioLegend Cat. No. 327019)
AB_2721718 (BioLegend Cat. No. 327020)
- Ig superfamily, MHC class II, heterodimeric transmembrane protein
B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs
- Peptide presentation
- CD3/TCR, CD4
- Cell Type
- Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
1. Levacher M, et al. 1990. Clin. Exp. Immunol. 81:177.
2. Terstappen L, et al. 1990. J. Leuk. Biol. 48:138.
3. Edwards J, et al. 1985. J. Immunol. 137:490.
4. van Es A, et al. 1984. Transplantation 37:65.
5. O'Doherty U, et al. 1994. Immunology 82:487.
6. Thomas R, et al. 1994. J. Immunol. 153:4016.
7. Grouard G, et al. 1996. Nature 384:364.
- Gene ID
- 3122 View all products for this Gene ID 3123 View all products for this Gene ID
- View information about HLA-DR on UniProt.org
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
|Purified anti-human HLA-DR||LN3||FC, IHC, IP|
|Biotin anti-human HLA-DR||LN3||FC, IHC|
|FITC anti-human HLA-DR||LN3||FC|
|PE anti-human HLA-DR||LN3||FC|
|Alexa Fluor® 488 anti-human HLA-DR||LN3||FC|
|Alexa Fluor® 647 anti-human HLA-DR||LN3||FC|
|Alexa Fluor® 700 anti-human HLA-DR||LN3||FC|
|Pacific Blue™ anti-human HLA-DR||LN3||FC|
|PE/Cyanine7 anti-human HLA-DR||LN3||FC|
|PerCP/Cyanine5.5 anti-human HLA-DR||LN3||FC|
|APC anti-human HLA-DR||LN3||FC|
|APC/Fire™ 750 anti-human HLA-DR||LN3||FC|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human HLA-DR
Biotin anti-human HLA-DR
FITC anti-human HLA-DR
PE anti-human HLA-DR
Alexa Fluor® 488 anti-human HLA-DR
Alexa Fluor® 647 anti-human HLA-DR
Alexa Fluor® 700 anti-human HLA-DR
Pacific Blue™ anti-human HLA-DR
PE/Cyanine7 anti-human HLA-DR
PerCP/Cyanine5.5 anti-human HLA-DR
APC anti-human HLA-DR
APC/Fire™ 750 anti-human HLA-DR