- CB9 (See other available formats)
- Regulatory Status
- Other Names
- Granzyme 1, Hanukah factor serine protease (HFSP), Cytotoxic T-lymphocyte-associated serine esterase 3 (CTLA-3)
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
Granzyme A is a 28 kD disulfide-linked homodimeric protein and the most abundant of the proteases occurring in CTL granules. It is homologous to other serine esterases, including other granyzmes, mast cell proteases, and neutrophil cathepsins. Granzyme B is thought to be a rapidly-acting apoptotic enzyme, while Granzyme A is slow acting. The CB9 monoclonal antibody recognizes human Granzyme A and has been shown to be useful for flow cytometry, immunoprecipitation, and immunohistochemistry (paraffin-embedded sections).Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Purified human Granzyme A
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining3 of formalin-fixed paraffin-embedded tissue sections, and immunoprecipitation2.
- Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
(PubMed link indicates BioLegend citation)
- Trimble L, et al. 1998. Blood 91:585.
- Beresford P, et al. 1997. P. Natl. Acad. Sci. USA 94:9285.
- Raqib R, et al. 2002. Infect. Immun. 70:3199.
- Chen H, et al. 2005. J. Immunol. 175:591.
- Product Citations
AB_2114394 (BioLegend Cat. No. 507215)
AB_2114393 (BioLegend Cat. No. 507216)
- Serine protease; disulfide-linked homodimer; 28 kD (Mammalian)
- Induction of caspase-independent cell death by apoptosis; release of NM23-H1 for single-strand DNA nicking
- Cell Sources
- Cytotoxic T cells, NK cells
- Cell Targets
- Intracellular targets lamins A, B cells, C, nucleosome assembly protein (NAP) SET cells, HMG2, Ape1/Ref-1, histones; cleaves SET:NM23-H1 complex to release NM23-H1
- Assisted by perforin
- Cell Type
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience
- Molecular Family
- Enzymes and Regulators, Proteases
- Antigen References
1. Brune J, et al. 1986. Nature 322:268.
2. Fan Z, et al. 2003. Nature Immunol. 4:145.
3. Fan Z, et al. 2003. Cell 112:659.
4. Masson D, et al. 1987. Cell 49:679.
- Gene ID
- 3001 View all products for this Gene ID
- View information about Granzyme A on UniProt.org
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
|Alexa Fluor® 594 anti-human Granzyme A||CB9||ICC, ICFC|
|APC anti-human Granzyme A||CB9||ICFC|
|PE/Cyanine7 anti-human Granzyme A||CB9||ICFC|
|FITC anti-human Granzyme A||CB9||ICFC|
|PE anti-human Granzyme A||CB9||ICFC|
|Purified anti-human Granzyme A||CB9||ICFC, ICC, IP, IHC-P|
|Pacific Blue™ anti-human Granzyme A||CB9||ICFC|
|Alexa Fluor® 700 anti-human Granzyme A||CB9||ICFC|
|Alexa Fluor® 488 anti-human Granzyme A||CB9||ICFC|
|Alexa Fluor® 647 anti-human Granzyme A||CB9||ICFC|
|PerCP/Cyanine5.5 anti-human Granzyme A||CB9||ICFC|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Alexa Fluor® 594 anti-human Granzyme A
APC anti-human Granzyme A
PE/Cyanine7 anti-human Granzyme A
FITC anti-human Granzyme A
PE anti-human Granzyme A
Purified anti-human Granzyme A
Pacific Blue™ anti-human Granzyme A
Alexa Fluor® 700 anti-human Granzyme A
Alexa Fluor® 488 anti-human Granzyme A
Alexa Fluor® 647 anti-human Granzyme A
PerCP/Cyanine5.5 anti-human Granzyme A