PerCP/Cyanine5.5 anti-human CD33, CD33, WM53

Analyte Specific Reagent. Analytical and performance characteristics are not established.

Clone: WM53
Workshop: IV M-505
Other Names: Siglec-3, gp67, p67
Isotype: Mouse IgG1, κ

WM53_PerCPCyanine55_CD33_Antibody_013019 — Typical results from human peripheral blood monocytes stained either with WM53 PerCP/Cyanine5.5 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).

Compare all formats See PerCP/Cyanine5.5 spectral data

Cat #	Size	Price	Save
983906	500 µL	¥129,360

Description

CD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.

Product Details

Technical data sheet

Product Details

Reactivity

Human

Formulation

Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions

Concentration

200 µg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C and protected from prolonged exposure to light. Do not freeze.

Application

Suggested for Flow Cytometry

Disclaimer

WARNINGS AND PRECAUTIONS

Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
Do not use this reagent beyond the expiration date stated on the label.
Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be indication of possible deterioration.
Avoid prolonged exposure of the reagent or stained cells to light.

Antigen Details

Antigen References

Favaloro EJ, et al. 1988. Br. J. Haematol. 69:163.
Freeman SD, et al. 1995. Blood 85:2005.

Documentation

Certificate of Analysis

Go To Top Version: 2 Revision Date: 07/08/2020

Analyte Specific Reagent. Analytical and performance characteristic are not established.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.