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PerCP/Cyanine5.5 anti-human CD275 (B7-H2, ICOSL) Antibody

Pricing & Availability
Clone
2D3 (See other available formats)
Regulatory Status
RUO
Other Names
CD275, ICOSL, B7H2, ICOS-L, B7RP-1, B7-RP1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
2D3_PerCPCyanine5dot5_CD275_Antibody_082019.png
Peripheral blood mononuclear cells stimulated with Recombinant human CD40L plus IL-4 (overnight) was stained with CD19 FITC and CD275 (B7-H2, ICOSL) (clone 2D3) PerCP/Cyanine5.5 (filled histogram), or mouse IgG2b, ? PerCP/Cyanine5.5 isotype control (open histogram).
  • 2D3_PerCPCyanine5dot5_CD275_Antibody_082019.png
    Peripheral blood mononuclear cells stimulated with Recombinant human CD40L plus IL-4 (overnight) was stained with CD19 FITC and CD275 (B7-H2, ICOSL) (clone 2D3) PerCP/Cyanine5.5 (filled histogram), or mouse IgG2b, ? PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Save
309417 25 tests ¥31,020
309418 100 tests ¥82,500
Description

B7-H2, a member of the B7 family and the immunoglobulin superfamily, is a 40 kD protein also known as B7RP-1, B7h, B7-H2, GL50 and ICOS Ligand. Human B7-H2 is expressed by B lymphocytes, activated monocytes/macrophages, and dendritic cells. B7-H2 binds to a CD28-like receptor, inducible costimulator molecule (ICOS, AILIM, CRP-1), which is expressed by activated T cells. The interaction of ICOS with B7-H2 plays an important role in the T cell costimulation pathway.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human B7H2-mIg fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Kurosawa S, et al. 2003. Am. J. Respir. Cell Mol. Biol. 28:563.
RRID
AB_2814156 (BioLegend Cat. No. 309417)
AB_2814157 (BioLegend Cat. No. 309418)

Antigen Details

Structure
Ig superfamily, B7 family, 40 kD
Distribution

B cells and dendritic cells

Function
Binds ICOS on antigen-activated T cells and mediates proliferation and cytokine production
Ligand/Receptor
ICOS
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Wang S, et al. 2002. J. Biol. Chem. 96:2808.
  2. Wong SC, et al. 2003. Blood 102:1831.
Gene ID
23308 View all products for this Gene ID
UniProt
View information about CD275 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 08/20/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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