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PE/Dazzle™ 594 anti-human/mouse Bcl-6 Antibody

Pricing & Availability
Clone
7D1 (See other available formats)
Regulatory Status
RUO
Other Names
B-cell lymphoma 6, BCL5, LAZ3, BCL6A, ZNF51, ZBTB27
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
7D1_PDZL594_BCL-6_Antibody_ICFC_080815
Burkitt’s lymphoma cell line, Ramos, was treated with the True-Nuclear™ Transcription Buffer Set, and stained with Bcl-6 (clone 7D1) PE/Dazzle™ 594 (filled histogram) or rat IgG2a, κ PE/Dazzle™ 594 isotype control (open histogram).
  • 7D1_PDZL594_BCL-6_Antibody_ICFC_080815
    Burkitt’s lymphoma cell line, Ramos, was treated with the True-Nuclear™ Transcription Buffer Set, and stained with Bcl-6 (clone 7D1) PE/Dazzle™ 594 (filled histogram) or rat IgG2a, κ PE/Dazzle™ 594 isotype control (open histogram).
Compare all formats See PE/Dazzle™ 594 spectral data
Cat # Size Price Save
358509 25 tests ¥34,980
358510 100 tests ¥81,400
Description

B-cell lymphoma 6 (Bcl-6), is an 80 kD homodimer, member of the BTB-POZ zinc finger family. It contains 1 BTB (POZ) domain and 6 C2H2-type zinc fingers. Bcl-6 is a transcriptional repressor, master regulator of germinal center reaction. On B cells, Bcl-6 induces proliferation, antibody class switch and affinity maturation, while inhibits its differentiation to plasma cells. On T cells, Bcl-6 induces its differentiation to TFH. This molecule is also expressed in some B cell lymphomas and breast cancer cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Sequence 226-398 of murine BCL6 fused with GST
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1,2,3.

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Vikstrom, et al. 2010. Science 330:1095. (WB)
  2. Kallies A, et al. 2011. Blood 117:1869. (WB)
  3. Lüthje K, et al. 2012. Nat. Immunol. 13:491. (WB)
  4. Tonti E, et al. 2012. J. Immunol. 188:3217. (ICFC)
RRID
AB_2566193 (BioLegend Cat. No. 358509)
AB_2566194 (BioLegend Cat. No. 358510)

Antigen Details

Structure
Member of the BTB-POZ zinc finger family, 80 kD, homodimer, 1 BTB (POZ) domain, 6 C2H2-type zinc fingers
Distribution

Germinal center B cells, follicular helper T cells (TFH), B cell lymphomas, breast cancer cells

Function
Transcription factor, master regulator of germinal center reaction
Interaction
BLIMP-1
Cell Type
B cells, Tfh
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Basso K and Dalla-Favera R. 2012. Immunol. Rev. 247:172.
2. Vinuesa CG, and Cyster JG, 2011. Immunity 35:671.
3. Kitano M, et al. 2011. Immunity 34:961.
4. Baumjohann D, et al. 2011. J. Immunol. 187:2089.
5. Johnston RJ, et al. 2009. Science. 325:1006.
6. Tran TH, et al. 2010. Cancer Res. 70:1711.

Gene ID
604 View all products for this Gene ID
UniProt
View information about Bcl-6 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/26/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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