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PE/Dazzle™ 594 anti-human IgM Antibody

Pricing & Availability
Clone
MHM-88 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin M
Isotype
Mouse IgG1, κ
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Product Citations
publications
MHM-88_PEDZL594_IgM_Antibody_FC_1_103015
Overnight cultured human peripheral blood mononuclear cells were stained with CD19 PE and anti-IgM (clone MHM-88) PE/Dazzle™ 594 (top) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (bottom). Data shown is gated on lymphocyte population.
  • MHM-88_PEDZL594_IgM_Antibody_FC_1_103015
    Overnight cultured human peripheral blood mononuclear cells were stained with CD19 PE and anti-IgM (clone MHM-88) PE/Dazzle™ 594 (top) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (bottom). Data shown is gated on lymphocyte population.
  • MHM-88_PEDZL594_IgM_Antibody_FC_2_103015
Compare all formats See PE/Dazzle™ 594 spectral data
Cat # Size Price Save
314529 25 tests ¥38,720
314530 100 tests ¥84,040
Description

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.

Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed
Product Citations
  1. Jennewein MF, et al. 2021. Cell Reports. 36(2):109353. PubMed
  2. Lindesmith LC, et al. 2020. Cell Mol Gastroenterol Hepatol. 0.586805556. PubMed
  3. Kreutmair S, et al. 2021. Immunity. . PubMed
  4. Cohen KW, et al. 2021. Cell Reports Medicine. 2(7):100354. PubMed
  5. Seydoux E, et al. 2020. Immunity. 53:98. PubMed
RRID
AB_2566482 (BioLegend Cat. No. 314529)
AB_2566483 (BioLegend Cat. No. 314530)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type
B cells
Biology Area
Immunology
Gene ID
3507 View all products for this Gene ID
UniProt
View information about IgM on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/23/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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