PE/Cyanine5 Streptavidin

Pricing & Availability
Other Names
SAv-PE/Cyanine5
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Product Citations
publications
SAV_PECy5_082808.jpg
C57BL/6 splenocytes stained with 145-2C11 biotin, followed by Sav-PE/Cyanine5
  • SAV_PECy5_082808.jpg
    C57BL/6 splenocytes stained with 145-2C11 biotin, followed by Sav-PE/Cyanine5
See PE/Cyanine5 spectral data
Cat # Size Price Save
405205 100 µg ¥20,000
Description

Streptavidin binds to biotin with high affinity. Streptavidin-PE/Cyanine5 is useful for detecting biotinylated antibodies. The excitation of PE/Cyanine5 by 488 nm laser light induces a light emission maximum of 670 nm.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with PE/Cyanine5 under optimal conditions.
Concentration
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-PE/Cyanine5 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested

Recommended Usage

Each lot of this Streptavidin-PE/Cyanine5 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular molar mass of streptavidin independent of any additional molecular mass that might be added by the PE/Cyanine5 conjugation. For immunofluorescent staining, the recommended use of this reagent is = 0.125 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Streptavidin-Phycoerythrin/Cyanine5 (PE/Cyanine5) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PE/Cyanine5 is 480 kD and Streptavidin alone is 52 kD.

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Wong KA, et al. 2008. J. Immunol. 15:180. PubMed
  2. Qi Q, et al. 2009. Blood PubMed
  3. Imada M, et al. 2009. Int immunol. PubMed
  4. Sawa Y, et al. 2009. Immunity. 30:447. PubMed
  5. Song R, et al. 2010. J. Virol. PubMed
  6. Abdel-Latif A, et al. 2010. Exp Hematol. 38:1131. PubMed
  7. De Monte L, et al. 2011. J. Exp Med. 208:469. PubMed
  8. Xu B, et al. 2012. PLoS One. 7:e44719. PubMed
  9. Su ML, et al. 2014. PLoS One. 9:98823. PubMed
  10. Chen A, et al. 2014. J Immunol. 193:35. PubMed
  11. Soni C, et al. 2014. J Immunol. 193:4400. PubMed
  12. Wong EB, et al. 2015. J Immunol. 194:4130. PubMed
Product Citations
  1. Rodriguez K 2008. J Immunol. 180:716. PubMed
  2. Imada M, et al. 2009. Int Immunol. 1.674305556. PubMed
  3. Qi Q, et al. 2009. Blood. 114:564. PubMed
  4. Song R, et al. 2010. J Virol. 84:6935. PubMed
  5. Monte L, et al. 2011. J Exp Med. 208:469. PubMed
  6. Xu B, et al. 2012. PLoS One. 7:e44719. PubMed
  7. Abdel-Latif A, et al. 2010. Exp Hematol. 38:1131. PubMed
  8. Sawa Y, et al. 2009. Immunity. 30:447. PubMed
  9. Su M, et al. 2014. PLoS One. 9:98823. PubMed
  10. Chen A, et al. 2014. J Immunol. 193:35. PubMed
  11. Soni C, et al. 2014. J Immunol. 193:4400. PubMed
  12. Wong E, et al. 2015. J Immunol. 194:4130. PubMed
  13. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  14. Cuenca M, et al. 2016. J Immunol. 196: 726 - 737. PubMed
  15. Domeier P, et al. 2016. J Exp Med. 213: 715 - 732. PubMed

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Go To Top Version: 4    Revision Date: 06/18/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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