PE anti-NF-κB p65 Antibody

Pricing & Availability
14G10A21 (See other available formats)
Regulatory Status
Other Names
Nuclear factor NF-kappa-B p65 subunit (NF-κB p65), Rel-A, transcription factor p65, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (NFKB3)
Mouse IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
Human lung adenocarcinoma epithelial cell line A549 was fixed and permeabilized with FOXP3 Fix/Perm Buffer Set, and then stained with NF-κB p65 (clone 14G10A21) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
  • 14G10A23_PE_NF-kBp65_Antibody_FC_111213.jpg
    Human lung adenocarcinoma epithelial cell line A549 was fixed and permeabilized with FOXP3 Fix/Perm Buffer Set, and then stained with NF-κB p65 (clone 14G10A21) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
Compare all formats See PE spectral data
Cat # Size Price Save
653003 25 tests ¥28,980
653004 100 tests ¥68,250

NF-κB p65 is a member of REL-like domain containing protein family, which forms a NF-κB complex with the other family members: NF-κB1 (p105/p50) or NF-κB2 (p100/p52). The NF-κB complex is inactivated and held in the cytoplasm by the NF-κB inhibitor IκB. In response to activation stimuli, IκB kinases (IKKs) phosphorylates IκB, resulting in degradation of IκB and liberation of NF-κB complex. The activated NF-κB complex translocates to the nucleus and binds to κB sites in the DNA of their target genes.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Partial human NF-κB p65 recombinant protein (451-551 aa)
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

AB_2562768 (BioLegend Cat. No. 653003)
AB_2562769 (BioLegend Cat. No. 653004)

Antigen Details

65 kD protein containing a Rel homology domain (RHD), an activation domain, and a 9aaTAD domain.

The inactivated NF-κB complex containing p65 subunit is bound to IκB and is localized to cytoplasm. Upon activation, I-κB is phosphorylated and degraded. The activated NF-κB complex is in turn translocated to the nucleus as a transcription factor.

NF-κB is a homodimeric or heterodimeric complex formed by the Rel-like domain-containing proteins. The most abundant form is p65 (RelA) - p50 (NF-κB1) heterodimer complex. The NF-κB complex is a ubiquitously expressed transcription factor which is involved in various biological functions, such as cell growth, tumorigenesis, differentiation, apoptosis, inflammation, and immune responses.
Interacts with NF-κB1 (p105/p50) or NF-κB2 (p100/p52) to form heterodimeric NF-κB complex. Interacts with HDAC1, HDAC3, and CBP. Interaction with MEN1 inhibits transactivation activity of NF-κB complex.
Cell Type
B cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Li Z, et al. 1997. Mol. Cell. Biol. 17:6184.
2. Saccani S, et al. 2004. J. Exp. Med. 200:107.
3. Nolan GP, et al. 1991. Cell 64:961.
4. Chen LF, et al. 2001. Science 293:1653.
5. Hansen SK, et al. 1994. Mol. Cell. Biol. 14:2593.
6. Chapman NR, et al. 2002. Biochem J. 366:459.

Gene ID
4790 View all products for this Gene ID
View information about NF-kappaB p65 on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All NF-κB p65 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-NF-κB p65 14G10A21 WB, IP, KO/KD-WB
PE anti-NF-κB p65 14G10A21 ICFC
APC anti-NF-κB p65 14G10A21 ICFC
Go To Top Version: 1    Revision Date: 11/19/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-NF-κB p65

  • PE anti-NF-κB p65

  • APC anti-NF-κB p65


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account