PE anti-mouse Lineage Cocktail with Isotype Ctrl

Pricing & Availability
145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70;
Regulatory Status
Other Names
Armenian Hamster IgG/Rat IgG2a/Rat IgG2b
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Product Citations
Cat # Size Price Save
133303 25 tests ¥91,350

The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The PE Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3ε, clone 145-2C11; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER-119/Erythroid cells, clone Ter-119. PE Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative control immunoglobulin.

Materials provided:
1. PE anti-mCD3ε/ PE anti-mGr-1/ PE anti-mCD11b/ PE anti-mCD45R(B220)/ PE anti-mTer-119, 25 tests
2. PE Armenian hamster IgG/ PE Rat IgG2b/ PE Rat IgG2a isotype controls, 25 tests

Materials not provided:
Cell Staining Buffer (Cat. No. 420201)

Product Details
Technical data sheet

Product Details

Verified Reactivity
This two-reagent set contains PE conjugated antibodies and isotype controls in optimal concentration for flow cytometric analysis.
The antibodies were purified by affinity chromatography, and conjugated with PE under optimal conditions.
Storage & Handling
It should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Morris SJ, et al. 1997. Development. 124(10):1929-1939
  2. Okada S, et al. 1992. Blood180 (12):3044-3050
  3. Spangrude GJ, et al. 1988. Science 241 (4861):58-62
  4. Spangrude GJ, et al. 1990. Exp Hematol. 18 (8):920-926
  5. Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed
  6. Ito R, et al. 2012. Exp Hematol. 40:953. PubMed.
Product Citations
  1. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  2. Lei F, et al. 2020. Proc Natl Acad Sci U S A. 117:23336. PubMed
  3. Antunes KH, et al. 2022. Front Immunol. 13:867022. PubMed
  4. Ito R, et al. 2012. Exp Hematol. 40:953. PubMed
  5. Rosu A, et al. 2021. Journal of Experimental Medicine. 218(3):. PubMed
  6. Verma A, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.26. PubMed
  7. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  8. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
AB_1595553 (BioLegend Cat. No. 133303)

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse Lineage Cocktail with Isotype Ctrl 145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70 FC
PE anti-mouse Lineage Cocktail with Isotype Ctrl 145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70 FC
Biotin anti-mouse Lineage Panel 145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70 FC
Go To Top Version: 2    Revision Date: 07/09/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-mouse Lineage Cocktail with Isotype Ctrl

  • PE anti-mouse Lineage Cocktail with Isotype Ctrl

  • Biotin anti-mouse Lineage Panel


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