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PE anti-mouse IgG2b Antibody

Pricing & Availability
Clone
RMG2b-1 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G2b
Isotype
Rat IgG1, κ
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Product Citations
publications
RMG2b-1_PE_IgG2b_Antibody_FC_050714
Human peripheral blood lymphocytes were stained with purified anti-human CD4 (clone OKT4, filled histogram) or purified mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG2b PE.
  • RMG2b-1_PE_IgG2b_Antibody_FC_050714
    Human peripheral blood lymphocytes were stained with purified anti-human CD4 (clone OKT4, filled histogram) or purified mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG2b PE.
Compare all formats See PE spectral data
Cat # Size Price Save
406707 25 µg ¥17,600
406708 100 µg ¥38,940
Description

The RMG2b-1 monoclonal antibody reacts with immunoglobulin G2b (IgG2b) in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. The RMG2b-1 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Kimura S, et al. 2020. Nat Commun. 0.620833333. PubMed
  2. Jagodinsky JC, et al. 2022. J Immunother Cancer. 10:. PubMed
  3. Hung PJ, et al. 2018. Mol Cell. 71:332. PubMed
  4. Delgado‐Benito V et al. 2018. Molecular cell. 72(4):636-649 . PubMed
  5. Sundaravinayagam D et al. 2019. Cell Rep. 28(6):1389-1399 . PubMed
  6. Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed
  7. Ma X, et al. 2020. Immunity. 53:1315. PubMed
  8. Vincendeau E, et al. 2022. Nat Commun. 13:3707. PubMed
  9. Clancy–Thompson E, et al. 2019. EMBO J. 38:e101260. PubMed
  10. Liang YH, et al. 2021. J Biomed Sci. 28:75. PubMed
RRID
AB_2563380 (BioLegend Cat. No. 406707)
AB_2563381 (BioLegend Cat. No. 406708)

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 02/12/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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