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PE anti-mouse CD49b (pan-NK cells) Antibody

Pricing & Availability
Clone
DX5 (See other available formats)
Regulatory Status
RUO
Other Names
α2 integrin, VLA-2 α chain, DX5, Integrin α2 chain, ITGA2
Isotype
Rat IgM, κ
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Product Citations
publications
DX5_PE_022208
C57BL/6 mouse splenocytes stained with NK1.1 (PK136) FITC and DX5 PE
  • DX5_PE_022208
    C57BL/6 mouse splenocytes stained with NK1.1 (PK136) FITC and DX5 PE
Compare all formats See PE spectral data
Cat # Size Price Save
108907 50 µg ¥21,720
108908 200 µg ¥43,580
Description

DX5 antigen has been recently characterized as CD49b. It is a 150 kD integrin α chain also known as α2 integrin, VLA-2 α chain, and integrin α2 chain. CD49b non-covalently associates with CD29 (β1 integrin) to form the CD49b/CD29 complex known as VLA-2, a receptor for collagen and laminin. CD49b is expressed on platelets, the majority of NK cells, NKT cells, and a small subset of CD8+ T cells (this population can be significantly increased following viral infection). DX5 is used for the identification and isolation of NK cells, and is especially useful for identifying NK cells in mice lacking the NK1.1 antigen.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-propagated NK1.1+ cells from C57BL/6 mice
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The DX5 clone detects cells expressing relatively high levels of CD49b and may not be useful for the detection of cells expressing low levels of CD49b. DX5 does not block NK cell killing or binding to collagen in vitro. Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity2 and immunohistochemical staining5 of formalin-fixed and paraffin-embedded tissue sections as well as immunohistochemical staining of acetone-fixed frozen sections10. The binding of DX5 antibody to splenic NK cells can be blocked by HMa2 antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Arase H, et al. 2001. J. Immunol. 167:1141. (FC)
  2. Sepulveda H, et al. 1999. J. Immunol. 163:1133.
  3. Norian LA and Allen PM. 2004. J. Immunol. 173:835. (FC)
  4. Andoniou CE, et al. 2005. Nature Immunology 6:1011.
  5. Oertelt S, et al. 2006. J. Immunol. 177:1655. (IHC) PubMed
  6. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370.
  7. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  8. Qui Q, et al. 2010. J. Immunol. 184:1681. (FC) PubMed
  9. Busche A, et al. 2011. J. Immunol. 186:2918. PubMed
  10. Kim HR, et al. 2011. Nephrology 16:545. (IHC) PubMed
  11. Seyoum B, et al. 2011. Vaccine. 29:8002. PubMed
  12. Younos IH, et al. 2012. Int Immunopharmacol. 13:245. PubMed
  13. Honjo K, et al. 2012. PNAS. PubMed.
  14. Huang HN, et al. 2013. Biomaterials. 34:10151. PubMed
Product Citations
  1. Glasner A,et al. 2017. Sci Rep.. 10.1038/s41598-017-12998-w. PubMed
  2. Xiang G, et al. 2022. Cell Death Dis. 13:451. PubMed
  3. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  4. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  5. Nautiyal J, et al. 2013. Development. 140:1079. PubMed
  6. Lu H, et al. 2021. Autophagy. 17:2511. PubMed
  7. Currier MA, et al. 2017. Oncotarget. 8:17412. PubMed
  8. Montes de Oca R, et al. 2021. Mol Cancer Ther. 20:1941. PubMed
  9. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  10. Li J, et al. 2019. JCI Insight. 5. PubMed
  11. Mingozzi F, et al. 2016. EMBO Mol Med. 8: 1039 - 1051. PubMed
  12. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  13. Steven A, et al. 2013. Mol Cancer Res. 11:1462. PubMed
  14. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  15. Suryawanshi RK, et al. 2021. Nat Commun. 12:6020. PubMed
  16. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  17. Hu Y, et al. 2022. J Nanobiotechnology. 20:417. PubMed
  18. Barsoumian HB, et al. 2020. J Immunother Cancer. 8:00. PubMed
  19. Vettorazzi S, et al. 2015. Nat Commun. 6: 7796. PubMed
  20. Textor A, et al. 2014. Cancer Res. 74:6769. PubMed
  21. Sauma D, et al. 2017. Immunol Res. 10.1007/s12026-017-8936-9. PubMed
  22. Shannon JP, et al. 2021. STAR Protoc. 2:100790. PubMed
  23. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  24. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  25. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
  26. Nakamura K, et al. 2013. Proc Natl Acad Sci U S A. 110:9421. PubMed
  27. Zhang W, et al. 2019. Mar Drugs. 0.845138889. PubMed
  28. Hu Y, et al. 2021. Cell Death Dis. 12:743. PubMed
  29. Li Y, Kaneda T 2016. Sci Rep. 6: 25077. PubMed
  30. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  31. Furuya Y, et al. 2013. Infect Immun . 81:3434. PubMed
  32. Brenndörfer E, et al. 2014. J Immunol. 192:1671. PubMed
  33. Wedekind MF, et al. 2021. iScience. 24(7):102759. PubMed
  34. Porrello A, et al. 2018. Nat Commun. 9:1988. PubMed
  35. Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed
  36. Hoyer FF, et al. 2020. Immunity. 51(5):899-914.e7.. PubMed
  37. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  38. Caetano MS, et al. 2019. Clin Cancer Res. 25:7576. PubMed
  39. Chen Y, et al. 2021. Braz J Med Biol Res. 54:e9570. PubMed
  40. Sinha S, et al. 2012. J Leukoc Biol. 92:713. PubMed
  41. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  42. Fan X, et al. 2014. PLoS One. 9:107638. PubMed
RRID
AB_313414 (BioLegend Cat. No. 108907)
AB_313415 (BioLegend Cat. No. 108908)

Antigen Details

Structure
Integrin α chain, 150 kD
Distribution

NK cells, subset of T cells

Function
Adhesion
Ligand/Receptor
Collagen, laminin
Cell Type
NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Arase H, et al. 2001. J. Immunol. 167:1141.
2. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
3. Sasaki K, et al. 2003. Int. Immunol. 15:701.
4. Inoue O, et al. 2003. J. Cell Biol. 160:769.

Gene ID
16398 View all products for this Gene ID
UniProt
View information about CD49b on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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