PE anti-mouse CD319 Antibody anti-CD319

Clone: 4G2 (See other available formats)
Regulatory Status: RUO
Other Names: 19A, CS1, CD319, CRACC, SLAMF7
Isotype: Rat IgG1, κ
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Product Citations: publications

4G2_PE_CD319_Antibody_1_100516 — C57BL/6 mouse splenocytes were stained with CD19 FITC and CD319 PE (top) or rat IgG1, κ isotype control PE (bottom)

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Cat #	Size	Price	Save
152005	25 µg	¥33,660
152006	100 µg	¥67,980

Description

CD319/CD319/CS1/CRACC/SLAMF7 is a 66 kD transmembrane protein belonging to the CD2 subset of the immunoglobulin superfamily; it is expressed on most NK cells, B cells, and subsets of T cells. CD319 activates immune responses in NK cells when coupled with SAP-family adaptors (SAP, EAT-2, ERT) and inhibits immune responses in the absence of SAP-family adaptors or when bound to SH-domain molecules.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CD319-Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2632676 (BioLegend Cat. No. 152005)
AB_2632677 (BioLegend Cat. No. 152006)

Antigen Details

Structure: Extracellular domain with one Ig like V-set domain and one Ig like C2-set domain, and an cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs.
Distribution: Spleen, lymph node, bone marrow, testis, thymus; NK cells, B cells, and subsets of T cells.
Function: Activates immune responses in NK cells when coupled with SAP-family adaptors (SAP, EAT-2, ERT).
Interaction: Interacts (via ITSM phosphorylated on Tyr-302) with SH2D1B, PTPN6/SHP-1, PTPN11/SHP-2, INPP5D/SHIP1, CSK and FYN, and SAP-family adaptors (SAP, EAT-2, ERT).
Cell Type: B cells, NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Li Y, et al. 2013. PLoS One 8(9):e76681.
2. Dongre P, et al. 2013. Mol. Immunol. 54:254-63.
3. Tovar V, et al. 2002. Immunogenetics 54:394.
4. Dong Z, et al. 2009. Nat. Immunol. 10:973.
Gene ID: 75345 View all products for this Gene ID
UniProt: View information about CD319 on UniProt.org

Documentation

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Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All CD319 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse CD319	4G2	FC,Direct ELISA,WB
PE anti-mouse CD319	4G2	FC
APC anti-mouse CD319	4G2	FC
TotalSeq™-C1265 anti-mouse CD319	4G2	PG
TotalSeq™-B1265 anti-mouse CD319	4G2	PG

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 10/05/2016

For Research Use Only. Not for diagnostic or therapeutic use.

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