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PE anti-human HLA-E Antibody

Pricing & Availability
Clone
3D12 (See other available formats)
Regulatory Status
RUO
Other Names
HLA class I histocompatibility antigen, alpha chain E, HLAE
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
3D12_PE_061909.jpg
Human peripheral blood lymphocytes stained with 3D12 PE
  • 3D12_PE_061909.jpg
    Human peripheral blood lymphocytes stained with 3D12 PE
Compare all formats See PE spectral data
Cat # Size Price Save
342603 25 tests ¥23,760
342604 100 tests ¥57,640
Description

HLA-E is a non-classical MHC class I (MHC-Ib) molecule. It is characterized by limited polymorphism and ubiquitous expression. HLA-E, as other MHC-I molecules, is heterodimerized with β2-microglobulin. HLA-E interacts with CD94/NKG2 receptor to regulate NK cell cytolysis activities.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Lee N, et al. 1998. Proc. Natl. Acad. Sci. USA. 95:5199.
  2. Wooden SL, et al. 2005. J. Immunol. 175:1383.
  3. Monaco EL, et al. 2008. J. Immunol. 181:5442.
  4. Corrah TW, et al. 2011. J. Virol. 85:3367. PubMed
Product Citations
  1. Nguyen MAT, et al. 2020. Immunohorizons. 0.465277778. PubMed
  2. Leite NC, et al. 2020. Cell Reports. 32(2):107894.. PubMed
  3. Rascle P, et al. 2021. iScience. 24:103109. PubMed
  4. Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
  5. Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed
  6. Abd Hamid M et al. 2019. Cancer Immunol Res. 7(8):1293-1306 . PubMed
  7. Liang YH, et al. 2021. J Biomed Sci. 28:75. PubMed
RRID
AB_1659250 (BioLegend Cat. No. 342603)
AB_1659249 (BioLegend Cat. No. 342604)

Antigen Details

Structure
HLA family, Ig superfamily, 42 kD
Distribution

Broadly expressed

Function
Mediate cell recognition by NK cells
Ligand/Receptor
CD94/NKG2
Biology Area
Immunology
Molecular Family
MHC Antigens
Antigen References

1. Sullivan LC, et al. 2008. Tissue Antigen. 72:415.
2. Koller BH, et al. 1988. J. Immunol. 141:897.

Gene ID
3133 View all products for this Gene ID
UniProt
View information about HLA-E on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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