PE anti-human CD162 Antibody

Pricing & Availability
KPL-1 (See other available formats)
Regulatory Status
Other Names
PSGL-1, p-selectin glycoprotein ligand-1
Mouse IgG1, κ
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Product Citations
Human peripheral blood lymphocytes stained with KPL-1 PE
  • KPL-1_PE_082808.jpg
    Human peripheral blood lymphocytes stained with KPL-1 PE
Compare all formats See PE spectral data
Cat # Size Price Save
328805 25 tests ¥23,100
328806 100 tests ¥50,820

CD162, also known as p-selectin glycoprotein ligand-1 (PSGL-1), is a 120 - 220 kD, mucin-like type I transmembrane glycoprotein. CD162 binds to CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin). The interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation. CD162 is expressed on neutrophils, monocytes, and most lymphocytes including NK and T cells but PSGL-1 stains B cells at significantly lower levels than other cell types.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Host Species
PSGL-1 transfected murine 300.19 pre B-cell line
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone KPL-1 is reported to recognize the tyrosine sulfation consensus motif of PSGL-11. Additional reported applications (for the relevant formats) include: Western Blot1, immunoprecipitation2, immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin embedded tissue sections1, blocks the recognition of PSGL-1 with P- and L-selectin1.

Application References

(PubMed link indicates BioLegend citation)
  1. Snapp KR, et al. 1998. Blood 91:154.
  2. Snapp KR, et al. 1998. J. Cell Biol. 142:263.
  3. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  4. Miyamura K, et al. 2011. J. Gen. Virol. 92:287. PubMed
  5. Cheng Q, et al. 2012. Lupus. 21:632. PubMed.
Product Citations
  1. Pereira JL, et al. 2021. Transl Oncol. 14:101125. PubMed
  2. Oggero S, et al. 2021. Journal of Extracellular Vesicles. 10(6):12084. PubMed
  3. Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
  4. Santos S, et al. 2011. Thromb Res. 127:105. PubMed
  5. Jamaly S, et al. 2018. J Thromb Haemost. 16(8):1546. PubMed
  6. Wang SS, et al. 2021. Cell Chemical Biology. 28(5):699-710.e5. PubMed
  7. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  8. Anderson NR, et al. 2019. Cell Adh Migr. 13:163. PubMed
AB_2185244 (BioLegend Cat. No. 328805)
AB_2185243 (BioLegend Cat. No. 328806)

Antigen Details


Neutrophils, monocytes, and most lymphocytes

Mediates the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation.
CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin)
Cell Type
Lymphocytes, Monocytes, Neutrophils
Biology Area
Cell Adhesion, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Snapp KR, et al. 1998. Blood 91:154.

Gene ID
6404 View all products for this Gene ID
View information about CD162 on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 04/22/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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