PE anti-human CD16 Antibody anti-CD16

Analyte Specific Reagent. Analytical and performance characteristics are not established.

Clone: 3G8
Workshop: V NK80
Other Names: FcγRIII
Isotype: Mouse IgG1, κ

3G8_PE_CD16_ASR_Antibody_051116 — Typical results from human peripheral blood lymphocytes stained either with 3G8 PE used at 5 µL/test (filled histogram) or with an isotype control (open histogram).

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Cat #	Size	Price	Save
980102	500 µL	¥69,300

Description

CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).

Product Details

Technical data sheet

Product Details

Reactivity

Human

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 0.2% (w/v) BSA (origin USA) and a stabilizer.

Preparation

The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.

Concentration

100 µg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

Suggested for Flow Cytometry

Disclaimer

WARNINGS AND PRECAUTIONS

Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
Do not use this reagent beyond the expiration date stated on the label.
Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be an indication of possible deterioration.
Avoid prolonged exposure of the reagent or stained cells to light.

Antigen Details

Antigen References: 1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275.
2. Stroncek D, et al. 1991. Blood 77:1572.
3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381.

Documentation

Certificate of Analysis

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Go To Top Version: 4 Revision Date: 07/08/2020

Analyte Specific Reagent. Analytical and performance characteristic are not established.

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