Pacific Blue™ anti-human TCR α/β Antibody

Pricing & Availability
IP26 (See other available formats)
Regulatory Status
Other Names
α/β TCR, TCR α/β
Mouse IgG1, κ
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Product Citations
Human peripheral blood lymphocytes stained with IP26 Pacific Blue™
  • IP26_PB_112409
    Human peripheral blood lymphocytes stained with IP26 Pacific Blue™
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Save
306715 25 µg ¥24,360
306716 100 µg ¥56,700

The IP26 antibody reacts with a monomorphic determinant of the α/β T-cell receptor, which is expressed on greater than 95% of normal peripheral blood CD3+ T cells. The α/β TCR recognizes a peptide bound to MHC leading to T-cell activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: T cell activation. When co-staining with anti-CD3, we recommend using clone UCHT1, since we have confirmed that IP26 does not compete with this clone.  Other anti-CD3 clones may compete out the binding of IP26.  

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. (FC)
  2. Joseph A, et al. 2008. J. Virol. 82:3078. (FC) PubMed
  3. Pinto JP, et al. 2010. Immunology. 130:217. PubMed
Product Citations
  1. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
  2. Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed
  3. Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed
  4. Carrion B, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
AB_1953256 (BioLegend Cat. No. 306715)
AB_1953257 (BioLegend Cat. No. 306716)

Antigen Details

Ig superfamily, with CD3 forms CD3/TCR complex

T cells, thymocytes

Antigen recognition, T cell activation
Peptide bound to MHC
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Adaptive Immunity, Immunology
Molecular Family
Antigen References

1. Marchalonis J, et al. 2002. J. Mol. Recognit. 15:260.

Gene ID
6955 View all products for this Gene ID 6957 View all products for this Gene ID
View information about TCR alpha/beta on

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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