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Pacific Blue™ anti-human/mouse Granzyme B Antibody

Pricing & Availability
Clone
GB11 (See other available formats)
Regulatory Status
RUO
Other Names
Granzyme-2, serine protease B, CCP1, Asp-ase, CTLA-1
Isotype
Mouse IgG1, κ
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Product Citations
publications
GB11_PB_GranzymeB_Antibody_FC_1_061013
Human peripheral blood lymphocytes were surface stained with CD8 APC and then intracellularly stained with Granzyme B (clone GB11) Pacific Blue™ (top) or mouse IgG1 Pacific Blue™ isotype control (bottom).
  • GB11_PB_GranzymeB_Antibody_FC_1_061013
    Human peripheral blood lymphocytes were surface stained with CD8 APC and then intracellularly stained with Granzyme B (clone GB11) Pacific Blue™ (top) or mouse IgG1 Pacific Blue™ isotype control (bottom).
  • GB11_PB_GranzymeB_Antibody_FC_2_061013
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Save
515407 25 tests ¥38,720
515408 100 tests ¥84,040
Description

Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Reported Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Wever PC, et al. 1998. Immunology. 93:383
  2. Arens R, et al. 2004. J. Exp. Med. 199:1595
  3. Lima M, et al. 2003. Am. J. Pathol. 163:763
  4. Wiede F, et al. 2014. J Autoimmun. 53:105. PubMed
  5. Baker GF, et al. 2014. Cancer Res. 74:5079. PubMed
  6. Nacer A, et al. 2014. PLoS Pathog. 10:1004528. PubMed
  7. Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed
Product Citations
  1. Moderzynski K, et al. 2016. PLoS Negl Trop Dis. . PubMed
  2. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  3. Chan LC, et al. 2019. J Clin Invest. 129:3324. PubMed
  4. Marquardt I, et al. 2022. Front Microbiol. 12:752549. PubMed
  5. Boal-Carvalho I, et al. 2020. EMBO Rep. 21:e50421. PubMed
  6. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  7. Xu W et al. 2019. Immunity. 50(4):1054-1068 . PubMed
  8. Remy-Ziller C, et al. 2017. Hum Vaccin Immunother. 10.1080/21645515.2017.1373921. PubMed
  9. Cheng L, et al. 2021. Cancer Immunol Immunother. Online ahead of print. PubMed
  10. Liang S, et al. 2022. Acta Pharm Sin B. 12:2494. PubMed
  11. Myers JA, et al. 2022. JCI Insight. :. PubMed
  12. Tan X, et al. 2021. Front Oncol. 11:768222. PubMed
  13. Böttcher JP, et al. 2018. Cell. 172:1022. PubMed
  14. Reyes RM, et al. 2021. Oncoimmunology. 10:2006529. PubMed
  15. Narasimhan PB, et al. 2018. PLoS Negl Trop Dis. 12:e0006404. PubMed
  16. Verma A, et al. 2021. Cell Rep. 37:109942. PubMed
  17. Ma X, et al. 2021. Cell Metabolism. 33(5):1001-1012.e5. PubMed
  18. Uceda-Castro R, et al. 2022. Cell Rep Med. 3:100821. PubMed
  19. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  20. Khan A, et al. 2015. J Virol. 89: 6619 - 6632. PubMed
  21. Moderzynski K, et al. 2017. PLoS Negl Trop Dis. 11(2):e0005404. PubMed
  22. Fan Z, et al. 2020. EMBO Mol Med. 12:e11571. PubMed
  23. Ren Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  24. Stotesbury C, et al. 2020. J Immunol. 204:1582. PubMed
  25. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  26. Barsoumian HB, et al. 2020. J Immunother Cancer. 8:00. PubMed
  27. Li C, et al. 2020. Immunity. 52(1):201-202. PubMed
  28. OConnor RA, et al. 2021. OncoImmunology. 10(1):1940675. PubMed
  29. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  30. LM S, et al. 2016. Cell Rep. 16(12): 3286-96. PubMed
  31. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  32. Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
  33. Di Mitri D, et al. 2019. Cell Rep. 28:2156. PubMed
  34. Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed
  35. Li E, et al. 2021. Front Immunol. 12:667177. PubMed
  36. Georg P, et al. 2022. Cell. 185:493. PubMed
  37. Burel J, et al. 2016. PLoS Negl Trop Dis. 10:e0005031. PubMed
  38. Younes AI, et al. 2021. Transl Oncol. 14:100983. PubMed
  39. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
  40. Pi J, et al. 2022. J Nanobiotechnology. 20:36. PubMed
  41. Si J, et al. 2020. Cancer Cell. 38(4):551-566.e11. PubMed
  42. Pahl JHW, et al. 2018. Cancer Immunol Res. 0.609027778. PubMed
  43. Wells AC et al. 2017. eLife. 6 pii: e26398. PubMed
  44. Spolski R et al. 2019. Elife. 8 pii: e45501. PubMed
  45. Peterfalvi A, et al. 2021. Int J Environ Res Public Health. 18:. PubMed
  46. Burger ML, et al. 2021. Cell. 184:4996. PubMed
  47. Dai X, et al. 2021. Molecular Cell. 81(11):2317-2331.e6. PubMed
  48. Glodde N et al. 2017. Immunity. 47(4):789-802 . PubMed
  49. Yang X, et al. 2021. Sci Rep. 11:13482. PubMed
  50. Laczkó D, et al. 2020. Immunity. 53:724. PubMed
  51. Wolf Y, et al. 2019. Cell. 179:219. PubMed
  52. Kuranda K, et al. 2018. J Clin Invest. 128:5267. PubMed
RRID
AB_2562195 (BioLegend Cat. No. 515407)
AB_2562196 (BioLegend Cat. No. 515408)

Antigen Details

Structure
32 kD serine protease
Distribution

Cytotoxic T-cells and NK cells, low on granulocytes, B cells and activated dendritic cells

Function
Induction of cell death and apoptosis
Ligand/Receptor
Mannose-6-phosphate receptor
Cell Type
B cells, Dendritic cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Enzymes and Regulators, Proteases
Antigen References

1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203
2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885
3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610
4. Wagner C, et al. 2008. Mol. Immunol. 45:1761

Gene ID
3002 View all products for this Gene ID 14939 View all products for this Gene ID 171528 View all products for this Gene ID
UniProt
View information about Granzyme B on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/12/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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