ELISA MAX™ Standard Set Mouse IL-10

Pricing & Availability
Other Names
IL-10 ELISA Kit
Ave. Rating
9 reviews
Product Citations
publications
IL-10_Mouse_ELISAMax_081712
  • IL-10_Mouse_ELISAMax_081712
Cat # Size Price Save
431411 5 plates ¥45,000
Description

The 10- and 20-plate sizes of this assay have been discontinued. The assay will remain available for purchase under the 5-plate catalog number. Please contact your BioLegend sales representative for bulk pricing quotations.

Mouse IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) due to its ability to inhibit cytokine production by TH1 clones. IL-10 is expressed in activated CD8+ and CD4+ T cells, activated monocytes, mast cells, and Ly-1 B cells. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The functions of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hypersensitivity response, and stimulation of the TH2 cell response, which results in elevated antibody production.


ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation.

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Mouse IL-10 ELISA MAX™ Capture Antibody (200X)
  • Mouse IL-10 ELISA MAX™ Detection Antibody (200X)
  • Mouse IL-10 Standard
  • Avidin-HRP (1000X)

Product Details

Reactivity
Mouse
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References

(PubMed link indicates BioLegend citation)
  1. Inoue S, et al. 2006. Cancer Research 66:7741.
  2. Lau AH, et al. 2006. J. Leukoc. Biol. 79:941.
  3. Kubo T, et al. 2009. J. Exp Med. 206:1971. PubMed
  4. Asai A, et al. 2012. Immunobiology. 217:711. PubMed
  5. Baca Jones C, et al. 2014. PLoS One. 9:90855. PubMed
Product Citations
  1. Asai A, et al. 2012. Immunobiology. 217:711. PubMed
  2. Jones C, et al. 2014. PLoS One. 9:90855. PubMed
  3. Murayama M, et al. 2015. Nat Commun. 6: 8483. PubMed
  4. Fenoy I, et al. 2015. Exp Parasitol. 154: 47-50. PubMed
  5. Fenoy IM, et al. 2015. Immunobiology. 22: 641-648. PubMed
  6. Egli N, et al. 2015. PLoS One. 10: 0142741. PubMed
  7. Singh N, et al. 2016. J Immunol. 196: 1108 - 1122. PubMed
  8. Croasdell A, et al. 2016. J Immunol. 196: 2742 - 2752. PubMed
  9. Andrade-Sousa A, et al. 2016. PLoS One. 11: 0163420. PubMed
  10. Th?nert R, et al. 2017. Nat Commun. 8:14268. PubMed
  11. Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
  12. Hu Y, et al. 2017. Biochim Biophys Acta. 10.1016/j.bbadis.2017.06.001. PubMed
Publication Library
Standard Range
31.3-2,000 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • Coating Buffer: Phosphate-Buffered Saline (PBS), pH 7.4
  • Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
  • PBS: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
  • Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
  • Wash bottle or automated microplate washer
  • TMB Substrate Solution - BioLegend Cat. No. 421101 is recommended.
  • Stop Solution (2 N H2SO4)
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Absorbent paper

Antigen Details

Cell Sources
Activated CD8+ T cells, Th0, Th2 subset of CD4+ T cells, Ly-1+ B cells, monocytes, macrophages, keratinocytes
Cell Type
Tregs
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Gene ID
16153 View all products for this Gene ID
UniProt
View information about IL-10 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 08/21/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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