MojoSort™ Human anti-PE Nanobeads

Pricing & Availability
Other Names
Human PE Positive Selection Kit, PE Positive Selection, PE Positive Enrichment, MojoSort™
Ave. Rating
Submit a Review
Product Citations
publications
Mojosort_Human_PE_Nanobeads_1_082317
PBMCs were prepared and stained with CD19 (clone HIB19) PE. PE-positive cells were then isolated using MojoSort™ Human anti-PE Nanobeads. Dead cells were excluded by 7-AAD.
  • Mojosort_Human_PE_Nanobeads_1_082317
    PBMCs were prepared and stained with CD19 (clone HIB19) PE. PE-positive cells were then isolated using MojoSort™ Human anti-PE Nanobeads. Dead cells were excluded by 7-AAD.
  • Mojosort_Human_PE_Nanobeads_2_082317
  • Mojosort_Human_PE_Nanobeads_3_082317
    PBMCs were prepared and stained with TCR Vγ9 (clone B3) PE. PE-positive cells were then isolated using MojoSort™ Human anti-PE Nanobeads. Dead cells were excluded by 7-AAD.
  • Mojosort_Human_PE_Nanobeads_4_082317
  • Mojosort_Human_PE_Nanobeads_5_082317
    PBMCs were prepared and stained with CD3 (clone UCHT1) PE. PE-positive cells were then isolated using MojoSort™ Human anti-PE Nanobeads. Dead cells were excluded by 7-AAD.
  • Mojosort_Human_PE_Nanobeads_6_082317
Cat # Size Price Save
480091 100 µl ¥34,000
480092 1 ml ¥100,000
Description

Human PE labeled PBMCs are either selected or depleted in a two-step separation process. The cells are first incubated with a BioLegend anti-human PE-conjugated antibody, followed by incubating with directly conjugated anti-PE Nanobeads. The PE+, magnetically labeled fraction is retained by the use of a magnetic separator. After collection or depletion of the PE+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc

Product Details
Technical data sheet

Kit Contents

Kit Contents

For Cat# 480091:

  • 1 vial containing 250 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution)
  • 1 vial containing 100 µl of Human anti-PE Nanobeads

For Cat# 480092:

  • 1 vial containing 1 ml of Human TruStain FcX™ (Fc Receptor Blocking Solution)
  • 1 vial containing 1 ml of Human anti-PE Nanobeads

Product Details

Reactivity
Human
Formulation
Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
Preparation
Conjugated nanobeads: Protein-coated magnetic beads.
Storage & Handling
All components should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

Volume of MojoSort™ Human anti-PE Nanobeads should be adjusted depending on starting percentage of cell type to be isolated. For 1x107 cells in 100 µl of buffer, use the following volumes:

Cell type

Cell Frequency in PBMCs

Volume of Nanobeads (for 1x107 cells in 100 µl of buffer)

CD3

50-60%

20 µl

CD4 (including monocytes)

50-55%

20 µl

CD8a (including low expressing cells)

15-20%

10 µl

CD19

5-10%

4 µl

TCR Vγ9

1-3%

2 µl

 

Always use 5 µL Human TruStain for 1x107 cells in 100 µl of buffer. If additional vials of TruStain are required, please order Cat: 422301 or 422302.  

 

For low frequency cells, pre-dilute the Nanobeads in order to pipette a minimum of 5 µl of any solution. For example, to isolate TCR Vγ9+ cells, pre-dilute 10 µl of PE Nanobeads in 40 µl of MojoSort™ buffer and add 10 µl of that dilution per sample. Avoid working with small volumes.

Application Notes

The MojoSort™ Human anti-PE Nanobeads are designed to be used with BioLegend PE (phycoerythrin) conjugated antibodies against human cells. It can be used as a two-step kit for the positive selection or depletion of PE+ labeled cells from peripheral blood mononuclear cells (PBMCs).

 

Antigen Details

Gene ID
NA

Related FAQs

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Go To Top Version: 0    Revision Date: 08/29/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Login / Register
Forgot your password? Reset password?
Create an Account