LEGENDplex™ Human Cytokine Panel 2 Detection Antibodies

Pricing & Availability
Regulatory Status
RUO
Other Names
Human Cytokine Panel 2 Mix and Match Subpanel, Multiplex assay, Flow cytometry, Inflammation, Flex set, Cytokines
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Product Citations
publications
Cat # Size Price  
740116 100 tests (3.5 mL) ¥50,000
Description

The LEGENDplex™ Human Cytokine Panel 2 Detection Antibodies product is intended for use with the Mix and Match Human Cytokine Panel 2 of products. The compatible corresponding reagents are as follows:

740399 (LEGENDplex™ Human Cytokine Panel 2 Standard)
741156 (LEGENDplex™ Buffer Set Q)
740103 (LEGENDplex™ Human TSLP Capture Bead A4, 13X)
740104 (LEGENDplex™ Human IL-1α Capture Bead A5, 13X)
740105 (LEGENDplex™ Human IL-1β Capture Bead A6, 13X)
740106 (LEGENDplex™ Human GM-CSF Capture Bead A7, 13X)
740107 (LEGENDplex™ Human IFN-α2 Capture Bead A8, 13X)
740108 (LEGENDplex™ Human IL-23 Capture Bead A10, 13X)
740109 (LEGENDplex™ Human IL-12 (p40) Capture Bead B2, 13X)
740110 (LEGENDplex™ Human IL-12 (p70) Capture Bead B3, 13X)
740111 (LEGENDplex™ Human IL-15 Capture Bead B4, 13X)
740112 (LEGENDplex™ Human IL-18 Capture Bead B5, 13X)
740113 (LEGENDplex™ Human IL-11 Capture Bead B6, 13X)
740114 (LEGENDplex™ Human IL-27 Capture Bead B7, 13X)
740115 (LEGENDplex™ Human IL-33 Capture Bead B9, 13X)

Product Details
Technical data sheet

Product Details

Reactivity
Human
Application

Multiplex


Learn more about LEGENDplex™ at biolegend.com/legendplex

Download the LEGENDplex™ software here.
Application Notes

Note: The target analyte IFN-a2 was previously listed as IFN-a. We have since determined that the antibody reagents used in this product are specific to only IFN-a2, and not all IFN-a isoforms.

Antigen Details

Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
NA

Related FAQs

If I don't have a vacuum, how do I remove the liquid from my plate?

If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.

Should I perform the assay with the filter plates or with V-bottom plates?

Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.

After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?

The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.

What is the shelf life of LEGENDplex™ kits?

LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.

Is special software required for data analysis?

Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.

Go To Top Version: 4    Revision Date: 09/09/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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