LEGEND MAX™ Mouse CCL11 ELISA Kit

Pricing & Availability
Other Names
Eotaxin, Eotaxin-1, SCYA11
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Product Citations
publications
CCL11_Mouse_ELISA_Kit_LegMax_093015
  • CCL11_Mouse_ELISA_Kit_LegMax_093015
Cat # Size Price Save
443907 1 Pre-coated Plate ¥79,000
Description

CCL11, also known as Eotaxin, is a small cytokine belonging to the CC chemokine family. The full length of CCL11 is 97 amino acids (aa) with a 23 aa signal peptide, which is cleaved to generate a 74 aa mature protein. The molecular mass of CCL11 is about 10 kD, and its gene is located on the q arm of chromosome 17. CCL11 expression has been found in normal tissues such as smooth muscle and small intestine. Endothelial cells, smooth muscle cells, epithelial cells, alveolar macrophages, and eosinophils are known to produce CCL11. Mouse CCL11 shares a 61% and 96% amino acid sequence identity to both human and rat, respectively.

CCL11 is produced when inflammatory cytokines, such as IL-5 and TNF-a, stimulate lung endothelial cells, fibroblasts, and smooth muscle cells in response to allergens. As a potent eosinophil chemoattractant, via binding to its major receptor, CCR3, CCL11 efficiently induces the accumulation of eosinophils, which is a response associated with allergic inflammation. Therefore, atopic dermatitis, asthma, and parasitic infections are a few of the eosinophilic inflammatory diseases directly related to CCL11. In addition, CCL11 induces the formation of blood vessels and other angiogenic responses which are accompanied by inflammatory infiltration and is also involved in regulation of tumor development.

The LEGEND MAX™ Mouse CCL11 ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a rat monoclonal anti-mouse CCL11 antibody. The detection antibody is a biotinylated goat polyclonal anti-mouse CCL11 antibody. This kit is specifically designed for the accurate quantitation of mouse CCL11 from cell culture supernatant, serum, plasma, and other biological fluids. This kit is analytically validated with ready-to-use reagents.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Anti-Mouse CCL11 Pre-coated 96 well Strip Microplate
  • Mouse CCL11 Detection Antibody
  • Mouse CCL11 Standard
  • Avidin-HRP
  • Assay Buffer B
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Reactivity
Mouse
Application

ELISA

Product Citations
  1. Maaske A, et al. 2016. J Control Release. 237: 14-22. PubMed
Publication Library
Sensitivity
0.825 ± 0.386 pg/mL
Standard Range
6.25 - 400 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Biology Area
Angiogenesis, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
20292 View all products for this Gene ID
UniProt
View information about CCL11 on UniProt.org

Related FAQs

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

Dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The Wash buffer is the same for all the current LEGEND MAX™ kits. All the part numbers on the Wash Buffer bottles in these kits should be the same. For ELISA MAX™ Deluxe and ELISA MAX™ Standard sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

In your LEGEND MAX™ ELISA Kits, there is a step that calls for a washing of the plates before even adding any sample to it. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The washings were designed to remove these components before you start the assay. If you do not do the washings, the effect on assay performance is negligible.

Go To Top Version: 1    Revision Date: 09/30/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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