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- Regulatory Status
- RUO
- Other Names
- Matrix Metallo proteinase 2, Gelatinase A, 72kD Type IV Collagenase, CLG4A
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- Product Citations
- publications
Matrix metalloproteinase 2 (MMP-2), also known as gelatinase A or 72 kD type IV collagenase, is a member of a broad family of MMPs, which are zinc-dependent enzymes that break down extracellular matrix proteins as part of their primary functions. MMP-2 is secreted in its 72 kD latent pro form from many cell types including neutrophils, monocytes, macrophages, endothelial cells, platelets, and tumor cells. It exists as a monomer and can form a homodimer that is known to regulate pro-MMP- 2 activation. When its pro-domain is cleaved by proteases such as MMP-14 and thrombin, it becomes the active 62 kD mature form. Activated MMP-2 degrades collagenase of the basement membrane and gelatin as well as several chemokines and cytokines. MMP-2 can also bind to other proteins, such as the tissue inhibitors of metalloproteinase (TIMPs). Specifically, TIMP-2 is able to bind to MMP-2 and MMP-14 to form a complex that regulates MMP-2 activation on the cell surface.
MMP-2 is closely related to MMP-9, although it only shares 46% amino acid sequence identity. Human MMP-2 shares significant {>95%) amino acid sequence identity with MMP-2 from other species, including nonhuman primates, equine, porcine, mouse, bovine, and rat.
MMP-2 plays important roles in vasculature remodeling, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP-2 is a potential biomarker for many diseases and conditions, as it is involved in tumor metastasis, inflammatory conditions, allergies, autoimmune diseases, and myocardial infarction.
The LEGEND MAX™ Human Total MMP-2 Sandwich Enzyme-Linked lmmunosorbent Assay (ELISA) kit includes a 96-well strip plate that is pre coated with a mouse monoclonal anti-human MMP-2 antibody. The Detection Antibody is a biotinylated rabbit polyclonal anti-human MMP-2 antibody. This kit is specifically designed to accurately measure MMP-2, including Pro MMP-2, Mature MMP-2, and Mature MMP-2/TIMP-2 complex. This kit has been analytically validated to assess MMP-2 levels in cell culture supernatant, serum, and heparin plasma samples with ready to-use reagents.
Kit Contents
- Kit Contents
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- Anti-human Total MMP-2 Pre-coated 96-well Strip Microplate
- Human Total MMP-2 Detection Antibody
- Human Total MMP-2 Standard
- Avidin-HRP
- Assay Buffer B
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
Product Details
- Verified Reactivity
- Human
- Application
-
ELISA
- Sensitivity
- 43.8 pg/mL ± 15.9 pg/mL for serum and heparin plasma samples, 193.5 pg/mL ± 138.5 pg/mL for cell culture supernatant samples.
- Standard Range
- 156 - 10,000 pg/mL for serum and heparin plasma samples, 625 - 40,000 pg/mL for cell culture supernatant samples.
- Materials Not Included
-
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
Antigen Details
- Biology Area
- Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience
- Molecular Family
- Enzymes and Regulators
- Gene ID
- 4313 View all products for this Gene ID
- UniProt
- View information about MMP-2 on UniProt.org
Related FAQs
- In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
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We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
- I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
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The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
- For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
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In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.
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