- Other Names
- Mesothelin, CAK1, and MSLN Pre-coated ELISA Kit
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|438607||1 Pre-coated Plate||¥83,000|
Human mesothelin, also known as CAK1 and MSLN, is a cell-surface protein expressed on normal mesothelial cells lining the pleura, pericardium, and peritoneum.
It is expressed as a 70 kD pre-cursor protein with a furin cleavage site, which produces two products: 1) megakaryocyte potentiating factor (MPF) (30 kD); and 2) mesothelin (40 kD). MPF is a cytokine which is released. Mesothelin, for the most part, is membrane-bound by a glycosyl-phosphatidyl inositol linkage and has three glycosylation sites. Soluble mesothelin is believed to result from proteolytic cleavage to release it from the membrane linkage or from alternative splicing of the mesothelin gene.
The normal function of mesothelin is not known. Mice knockouts produced no obvious phenotypes with normal growth and reproductive parameters.
However mesothelin is overexpressed by cancer cells, particularly in mesotheliomas, ovarian adenocarcinomas, pancreatic adenocarcinomas, squamous cell carcinomas, and lung cancers. It binds to MUC16/CA-125, a cell surface mucin and ovarian cancer biomarker, and has been speculated to be involved in peritoneal dissemination of ovarian cancer cells. Mesothelin has also been shown to be elevated in the serum of cancer patients compared to normal. As such, it has become the focus of cancer research in the areas of diagnostics, immunotherapy, and cancer vaccinology. For example, SS1P is a recombinant immunotoxin that targets mesothelin-expressing cancer cells. Its anti-mesothelin Fv domain confers specificity, while its Pseudomonas exotoxin domain results in cell death. This potential drug has already finished stage I clinical trials.
BioLegend's LEGEND MAX™ Human Mesothelin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a human mesothelin-specific monoclonal antibody is pre-coated on a 96-well strip-well plate.
BioLegend's LEGEND MAX™ Human Mesothelin ELISA Kit is specifically designed for the accurate quantitation of Human Mesothelin from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.
- Kit Contents
- Anti-Human Mesothelin Pre-coated 96-well Strip Microplate
- Human Mesothelin Dectection Antibody
- Human Mesothelin Standard
- Avidin-HRP A
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- 35.1 pg/mL
- Standard Range
- 78.1-5,000 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Plate Shaker
- Polypropylene vials
- For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.
- I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
- In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.