LEAF™ Purified anti-mouse/rat/human MCP-1 Antibody

Pricing & Availability
Clone
2H5 (See other available formats)
Other Names
Monocyte chemoattractant protein-1, Monocyte chemoattractant and activating factor (MCAF), JE, Small inducible cytokine A2 (SCYA2), HC-11, P6, Smooth muscle cell chemotactic factor (SMC-CF), CCL2
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
Cat # Size Price Save
505905 50 µg ¥19,000
505906 500 µg ¥56,000
This product is scheduled for discontinuation as a part of our ongoing LEAF™ to Ultra-LEAF™ antibody transition. The equivalent Ultra-LEAF™ product may be available at comparable or significantly lower price. Please use the availability checker to determine current availability, or contact technical service for further inquiries.
Description

Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 2H5 antibody reacts with mouse, rat, and human MCP-1. The 2H5 antibody can neutralize the bioactivity of natural or recombinant MCP-1.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Rat, Human, Cross-Reactivity: Rhesus
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
CHO-expressed, recombinant mouse MCP-1
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

ELISA Capture - Quality tested
ELISPOT Capture, Neut, WB, IHC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 5-10 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate.

Application Notes

ELISA or ELISPOT Capture1: The Ultra-LEAF™ 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody (Cat. No. 506002) as the detecting antibody for the detection of mouse MCP-1. The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 5D3-F7 antibody (Cat. No. 502609) as the detecting antibody for the detection of human MCP-1.
Flow Cytometry2: The fluorochrome-labeled 2H5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP-1 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization1,4,6: The Ultra-LEAF™ purified antibody (Cat. Nos. 505911 & 505912) is recommended for neutralization.
Additional reported applications (for the relevant formats) include: Western blotting3, immunohistochemistry of paraformaldehyde-fixed, saponin-treated frozen tissue sections5, and acetone-fixed frozen tissue sections7.

Application References

(PubMed link indicates BioLegend citation)
  1. Luo, Y., et al. 1994. J. Immunol. 153:3708.
  2. Zhang, Y., et al. 2002. J. Immunol. 168:3088.
  3. Luo, Y., et al. 1999 J. Immunol. 163:3985.
  4. Morrison, B. E., et al. 2003 J. Clin. Invest. 112:1862.
  5. Hancock, W. W., et al. 1997 Transplantation 64:696.
  6. Yu, R., et al. 2006 Obesity 14:1353.
  7. Taal MW, et al. 2001. Am. J. Physiol. Renal. Physiol. 280:F343.
  8. Wan W, et al. 2015. Cardiovasc Res. 106:478. PubMed
Product Citations
  1. Ziraldo C, et al. 2013. PLoS One. 8:79804. PubMed
  2. Kobayashi K, et al. 2018. Hepatol Commun. 2:230. PubMed
Publication Library
RRID
AB_315411 (BioLegend Cat. No. 505905)
AB_2071552 (BioLegend Cat. No. 505906)

Antigen Details

Structure
CC chemokine; dimer; 13.8 kD (Mammalian)
Bioactivity
Chemoattractant; regulates adhesion molecule expression, cytokine production in monocytes; proliferation/activation of CC-chemokine activated killer cells
Cell Sources
Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells
Cell Targets
Monocytes, basophils, T cells, NK cells, immature dendritic cells
Receptors
CCR2; CCR5; CCR10; D6; possibly L-CCR
Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Bischoff, S., et al. 1992. J. Exp. Med. 175:1271.
3. Charo, I., et al. 1994. P. Natl. Acad. Sci. USA 91:2752.
4. Jiang, Y., et al. 1992. J. Immunol. 148:2423.

Regulation
Upregulated by IL-1, TNF-α, PDGF, TGF-β
Gene ID
20296 View all products for this Gene ID 24770 View all products for this Gene ID 6347 View all products for this Gene ID
UniProt
View information about CCL2 on UniProt.org

Related FAQs

Does BioLegend test each ULEAF™ antibody by functional assay?

No, BioLegend does not test ULEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each ULEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 5    Revision Date: 09/02/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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