Keratin 15 Polyclonal Antibody, Purified

Pricing & Availability
Clone
Poly18339 (See other available formats)
Other Names
Keratin, type I cytoskeletal 15, CK-15, cytokeratin-15, keratin-15, beta, keratin-15, basic
Previously
Covance Catalog# PCK-153P
Isotype
Chicken Polyclonal
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Product Citations
publications
Poly18339_Purified_Keratin_15_Antibody_IHC_022715
Immunohistochemistry staining with anti-K15 (clone Poly18339) showing strong cytoplasmic staining of the basal cells of normal human skin epidermis.
  • Poly18339_Purified_Keratin_15_Antibody_IHC_022715
    Immunohistochemistry staining with anti-K15 (clone Poly18339) showing strong cytoplasmic staining of the basal cells of normal human skin epidermis.
Instructions for Use (pdf)

Product Details

Intended Use
In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.

The polyclonal antibody Poly18339 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of Keratin 15. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Reactivity
Human, Mouse, Primate
Antibody Type
Polyclonal
Host Species
Chicken
Immunogen
This monospecific polyclonal antibody was raised in chickens against a peptide sequence derived from the C-terminus of the human keratin 15 protein, CDGQVVSSHKREI.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by IgY affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
When stored at ≤ -20°C, product is stable until the date shown on the label. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. If thawed and stored at 2-8°C, product is stable for 24 months from the date of thaw or until the expiry date on the label, whichever comes first.
Application

IHC

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
IHC: 1:500 with a biotin based detection system such as biotinylated anti-chicken immunoglobulin and USA Ultra Streptavidin Detection (Cat. No. 931001).

Tissue Sections: Paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Citrate Buffer Retrieval solution (Cat. No. 928502, 928602).
Incubation: 60 minutes at room temperature

Precautions for Use: Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.

Application Notes

This antibody is effective in immunohistochemistry (IHC).

Application References

(PubMed link indicates BioLegend citation)
  1. Liu Y, Lyle S, Yang Z, Cotsarelis G. Keratin 15 promoter targets putative epithelial stem cells in the hair follicle bulge. J Invest Dermatol 121(5):963-8, 2003. [IHC]
  2. Jih DM, Lyle S, Elenitsas R, Elder DE, Cotsarelis G. Cytokeratin 15 expression in trichoepitheliomas and a subset of basal cell carcinomas suggests they originate from hair follicle stem cells. J Cutan Pathol 26(3):113-118, 1999.
  3. Leube RE, Bader BL, Bosch FX, Zimbelmann R, Achtstaetter T, Franke WW. Molecular characterization and expression of the stratification-related cytokeratins 4 and 15. J Cell Biol 106(4):1249-1261, 1988.
Product Citations
  1. Callejas-Valera J, et al. 2016. Carcinogenesis. 37: 1014 - 1025. PubMed
  2. Miyashita H, et al. 2017. Sci Rep. 7:43557. PubMed
  3. Choo MK, et al. 2018. Sci Signal. 11:551. PubMed
Publication Library

Antigen Details

Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
3866 View all products for this Gene ID
UniProt
View information about Keratin 15 on UniProt.org

Related FAQs

Does BioLegend manufacture antibodies at GMP grade?

BioLegend antibodies and reagents that carry the IVD or ASR designation are manufactured in a registered GMP facility, while our other products are manufactured under ISO 13485 certification. Both environments provide high quality products. To learn more about our quality standards visit our Quality Control page at: https://www.biolegend.com/QC.

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Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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