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HRP anti-His Tag Antibody

Pricing & Availability
Clone
J099B12 (See other available formats)
Regulatory Status
RUO
Other Names
Histidine Tag
Isotype
Mouse IgG1, κ
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Product Citations
publications
J099B12_His_Tag_Antibody_ELISA_101614
A 96 well plate is coated with 100 µL of a His-tagged recombinant protein at 100 ng/mL. The coated protein is detected with HRP-conjugated anti-His tag antibody at concentration ranged from 1 to 0.008 µg/mL.
  • J099B12_His_Tag_Antibody_ELISA_101614
    A 96 well plate is coated with 100 µL of a His-tagged recombinant protein at 100 ng/mL. The coated protein is detected with HRP-conjugated anti-His tag antibody at concentration ranged from 1 to 0.008 µg/mL.
  • J099B12_His_Tag_Antibody_WB_101614
    A His-tagged recombinant protein is diluted in cell-culture medium. The diluted protein is loaded to 12% SDS PAGE gel in amount of 40, 20, and 10 ng. After electrophoresis, the His-tagged recombinant protein is detected with 0.3 µg/mL of the HRP-conjugated anti-His tag monoclonal antibody.
Compare all formats
Cat # Size Price Save
652503 25 µg ¥24,640
652504 100 µg ¥90,420
Description

This antibody was raised against linear 8XHis tag. It reacts with His tag protein, either C, N, or internal His6 and up repeats. It can be used for Western blotting to detect linear His repeat (His6 or more).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated His-tag peptide
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solution with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light. Avoid exposure to sodium azide.
Application

ELISA - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by direct ELISA assay. For direct ELISA, a concentration range of 0.01 - 1.0 µg per ml is recommended. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. Avoid using diluent solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the end-user titrate each lot of reagents for optimal performance.

Application References

(PubMed link indicates BioLegend citation)
  1. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
  2. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
Product Citations
  1. Hay CE, et al. 2020. J Pharmacol Exp Ther. 16:374. PubMed
  2. Bojadzic D, et al. 2021. Front Pharmacol. 11:600372. PubMed
  3. Bojadzic D, et al. 2021. ACS Infect Dis. 7:1519. PubMed
  4. Wang HC, et al. 2020. Antimicrob Agents Chemother. 65:. PubMed
  5. Zhang D, et al. 2020. Oncoimmunology. 9:1744921. PubMed
  6. Chen X, et al. 2021. Nat Commun. 12:5506. PubMed
  7. Fan Z, et al. 2020. EMBO Mol Med. 12:e11571. PubMed
  8. Zhao Y, et al. 2019. Cancer Biol Ther. 0.925. PubMed
  9. Zhang X, et al. 2021. Nat Chem. 13:626. PubMed
  10. Chen X, et al. 2020. bioRxiv. . PubMed
  11. Houben B, et al. 2020. EMBO J. e102864:39. PubMed
  12. Harrasser M, et al. 2022. Breast Cancer Res. 24:39. PubMed
  13. Hay CE, et al. 2018. PLoS One. 13:29. PubMed
RRID
AB_2734520 (BioLegend Cat. No. 652503)
AB_2563555 (BioLegend Cat. No. 652504)

Antigen Details

Function
Recombinant poly-histidine tag detection
Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about His Tag on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 09/21/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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