GoInVivo™ Purified anti-human CD366 (Tim-3) Antibody

Pricing & Availability
F38-2E2 (See other available formats)
Regulatory Status
HCDM listed
Other Names
T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366
Mouse IgG1, κ
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Product Citations
Cat # Size Price Save
345038 5 mg ¥142,560
345039 25 mg お問い合わせください
345040 50 mg お問い合わせください
345035 100 mg お問い合わせください
345036 500 mg お問い合わせください
345037 1 g お問い合わせください

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on activated T cells (preferentially on Th1 cells, monocytes/macrophages, and dendritic cells). Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Human Tim-3 fusion protein
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <1.0 EU/mg of the protein. (<0.1 pg/µg of the protein) as determined by the LAL test.
The GoInVivo™ antibody was purified by affinity chromatography.
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.

FC - Quality tested
Costim, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment.

Application Notes

GoInVivo™ products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo™ Webpage.

Additional reported applications (for relevant formats of this clone) include: costimulation1 (clone 2E2 has been shown to enhance T-cell receptor mediated activation and cytokine secretion) and blocking2,3.

Application References

(PubMed link indicates BioLegend citation)
  1. Hastings WD, et al. 2009. Eur. J. Immunol. 39:2492. (Costim)
  2. Jones RB, et al. 2008. J. Exp. Med. 205:2763. (Block)
  3. Klibi J, et al 2009. Blood 113:1957. (FC, Block)
Product Citations
  1. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  2. Meng Q, et al. 2021. J Immunother Cancer. 9:. PubMed
AB_2566293 (BioLegend Cat. No. 345038)
AB_2566294 (BioLegend Cat. No. 345039)
AB_2566295 (BioLegend Cat. No. 345040)
AB_2566086 (BioLegend Cat. No. 345035)
AB_2566087 (BioLegend Cat. No. 345036)
AB_2566088 (BioLegend Cat. No. 345037)

Antigen Details

Transmembrane protein containing immunoglobulin domain and mucin-like domain; can exist as a soluble form lacking mucin and transmembrane domains

Activated T cells, preferentially on Th1 cells, monocytes, dendritic cells

Plays a role in regulating macrophage activation, T cell apoptosis and immune tolerance
Cell Type
Dendritic cells, Monocytes, T cells, Th1, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Hafler DA and Kuchroo V. 2008. J. Exp. Med. 205:2699.
2. Zhu C, et al. 2005. Nat. Immunol. 6:1245.
3. Wang F, et al. 2009. Immunobiology 214:342.

Gene ID
84868 View all products for this Gene ID
View information about CD366 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD366 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD366 (Tim-3) F38-2E2 FC
PE anti-human CD366 (Tim-3) F38-2E2 FC
Brilliant Violet 421™ anti-human CD366 (Tim-3) F38-2E2 FC
Ultra-LEAF™ Purified anti-human CD366 (Tim-3) F38-2E2 FC,Costim,Block
APC anti-human CD366 (Tim-3) F38-2E2 FC
PE/Cyanine7 anti-human CD366 (Tim-3) F38-2E2 FC
PerCP/Cyanine5.5 anti-human CD366 (Tim-3) F38-2E2 FC
Brilliant Violet 605™ anti-human CD366 (Tim-3) F38-2E2 FC
FITC anti-human CD366 (Tim-3) F38-2E2 FC
Purified anti-human CD366 (Tim-3) (Maxpar® Ready) F38-2E2 FC,CyTOF®
Brilliant Violet 711™ anti-human CD366 (Tim-3) F38-2E2 FC
APC/Cyanine7 anti-human CD366 (Tim-3) F38-2E2 FC
Brilliant Violet 785™ anti-human CD366 (Tim-3) F38-2E2 FC
Brilliant Violet 650™ anti-human CD366 (Tim-3) F38-2E2 FC
Brilliant Violet 510™ anti-human CD366 (Tim-3) F38-2E2 FC
PE/Dazzle™ 594 anti-human CD366 (Tim-3) F38-2E2 FC
GoInVivo™ Purified anti-human CD366 (Tim-3) F38-2E2 FC,Costim,Block
APC/Fire™ 750 anti-human CD366 (Tim-3) F38-2E2 FC
Pacific Blue™ anti-human CD366 (Tim-3) F38-2E2 FC
Biotin anti-human CD366 (Tim-3) F38-2E2 FC
TotalSeq™-A0169 anti-human CD366 (Tim-3) F38-2E2 PG
TotalSeq™-C0169 anti-human CD366 (Tim-3) F38-2E2 PG
PE/Cyanine5 anti-human CD366 (Tim-3) F38-2E2 FC
TotalSeq™-B0169 anti-human CD366 (Tim-3) F38-2E2 PG
Brilliant Violet 750™ anti-human CD366 (Tim-3) Antibody F38-2E2 FC
TotalSeq™-D0169 anti-human CD366 (Tim-3) F38-2E2 PG
PE/Fire™ 810 anti-human CD366 (Tim-3) F38-2E2 FC
PE/Fire™ 640 anti-human CD366 (Tim-3) F38-2E2 FC
Go To Top Version: 4    Revision Date: 07/03/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD366 (Tim-3)

  • PE anti-human CD366 (Tim-3)

  • Brilliant Violet 421™ anti-human CD366 (Tim-3)

  • Ultra-LEAF™ Purified anti-human CD366 (Tim-3)

  • APC anti-human CD366 (Tim-3)

  • PE/Cyanine7 anti-human CD366 (Tim-3)

  • PerCP/Cyanine5.5 anti-human CD366 (Tim-3)

  • Brilliant Violet 605™ anti-human CD366 (Tim-3)

  • FITC anti-human CD366 (Tim-3)

  • Purified anti-human CD366 (Tim-3) (Maxpar® Ready)

  • Brilliant Violet 711™ anti-human CD366 (Tim-3)

  • APC/Cyanine7 anti-human CD366 (Tim-3)

  • Brilliant Violet 785™ anti-human CD366 (Tim-3)

  • Brilliant Violet 650™ anti-human CD366 (Tim-3)

  • Brilliant Violet 510™ anti-human CD366 (Tim-3)

  • PE/Dazzle™ 594 anti-human CD366 (Tim-3)

  • GoInVivo™ Purified anti-human CD366 (Tim-3)

  • APC/Fire™ 750 anti-human CD366 (Tim-3)

  • Pacific Blue™ anti-human CD366 (Tim-3)

  • Biotin anti-human CD366 (Tim-3)

  • TotalSeq™-A0169 anti-human CD366 (Tim-3)

  • TotalSeq™-C0169 anti-human CD366 (Tim-3)

  • PE/Cyanine5 anti-human CD366 (Tim-3)

  • TotalSeq™-B0169 anti-human CD366 (Tim-3)

  • Brilliant Violet 750™ anti-human CD366 (Tim-3) Antibody

  • TotalSeq™-D0169 anti-human CD366 (Tim-3)

  • PE/Fire™ 810 anti-human CD366 (Tim-3)

  • PE/Fire™ 640 anti-human CD366 (Tim-3)


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