Fixation Buffer

Pricing & Availability
Other Names
Fixative, Paraformaldehyde
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420801 100 mL ¥10,000
Description

Fixation Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Fixation Buffer is used to fix cells prior to permeabilization using Permeabilization Wash Buffer (Cat. No. 421002). BioLegend's Fixation Buffer has been formulated with prescreened paraformaldehyde with low background, thus producing the greatest signal to noise ratio.

Product Details
Technical data sheet

Product Details

Storage & Handling
This buffer solution should be stored between 2°C and 8°C.
Application

ICFC - Quality tested
ICC

Recommended Usage

For cell fixation, use 0.5 ml fixation buffer per tube and leave it in the dark for 20 minutes at room temperature. It is recommended that the reagent be titrated for optimal performance for each application. For the fixation procedure, please refer to the "Intracellular Cytokine Staining Protocol" under "Support" on BioLegend's website.
Caution: This buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contacts.

Application Notes

This 1X PBS solution contains 4% paraformaldehyde, which is toxic and is a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided.

Additional Product Notes

View more applications data using this product to stain Veri-Cells™ lyophilized control cells and to perform phospho-flow in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Kang YJ, et al. 2007. Nature Immunol. 8:601.
  2. Kenna TJ, et al. 2010. J. Immunol. 184:598. PubMed
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  4. del Rio ML, et al. 2011.Transplantation. 92:1085. PubMed
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  6. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  7. Haberthur K, et al. 2013. J Virol. 87:11751. PubMed
  8. Busskamp V, et al. 2014. Mol Syst Biol. 10:760. PubMed
Product Citations
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  2. Kuo T, et al. 2017. Oncogene. 10.1038/onc.2017.156. PubMed
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  4. Melcher M, et al. 2017. Stem Cell Res Ther . 10.1186/s13287-017-0601-7. PubMed
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  10. Gensterblum E,et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.09.011. PubMed
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  13. Bajpai G, et al. 2018. Nat Med. 24:1234. PubMed
  14. Nightingale K, et al. 2018. Cell Host Microbe. 24:447. PubMed
  15. Salybekov AA, et al. 2018. PLoS One. 13:e0203244. PubMed
  16. Spitzer SO, et al. 2019. Neuron. 101:459. PubMed
  17. Martinez–Usatorre A, et al. 2019. Cancer Immunol Res. 0.995138889. PubMed
  18. Jimenez RV, et al. 2019. Front Immunol. 1.932638889. PubMed
  19. Chen YF, et al. 2019. J Biomed Sci. 26:85. PubMed
  20. Angiari S, et al. 2020. Cell Metab. 31:391. PubMed
  21. Klepsch V, et al. 2020. Cell Commun Signal. 18:08. PubMed
  22. Martinez-Usatorre A, et al. 2020. Front Immunol. 11:340. PubMed
  23. Haryadi L, et al. 2019. Vet World. 12:1529. PubMed
  24. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  25. Kundu K, et al. 2019. Cancer Immunol Res. 1.069444444. PubMed
  26. Lownik JC, et al. 2020. Biochem Biophys Rep. 24:100803. PubMed
  27. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  28. Sullivan B, et al. 2010. Am J Pathol. 177:2837. PubMed
  29. Kenna T, et al. 2010. J Immunol. 184:598. PubMed
  30. Rio M, et al. 2012. J Immunol. 188:4885. PubMed
  31. Rio M, et al. 2011. Transplantation. 92:1085. PubMed
  32. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  33. Haberthur K, et al. 2013. J Virol. 87:11751. PubMed
  34. Busskamp V, et al. 2014. Mol Syst Biol. 10:760. PubMed
  35. Wright A, et al. 2015. Immunobiology. 220: 859-864. PubMed
  36. Clancy-Thompson E, et al. 2015. Cancer Immunol Res. 3: 956-967. PubMed
  37. Ede J, et al. 2015. Toxicol Sci. 148: 108 - 120. PubMed
  38. Glaser K, et al. 2016. PLoS One. 11: 0146898. PubMed
  39. Guye P, et al. 2016. Nat Commun. 7:10243. PubMed
  40. Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed
  41. Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed
  42. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  43. Ikebuchi R, et al. 2016. Sci Rep. 6:35002. PubMed
  44. Bekeschus S, et al. 2016. J Leukoc Biol. 100: 791 - 799. PubMed
  45. Liu X, et al. 2016. Sci Rep. 6:36722. PubMed
  46. Yokota-Nakatsuma A, et al. 2016. Sci Rep. 6:37914. PubMed
  47. Wang L, et al. 2016. Oncogene. 10.1038/onc.2016.380. PubMed
  48. Lamb C, et al. 2016. J Crohns Colitis. 10.1093/ecco-jcc/jjw189. PubMed
  49. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  50. Cho S, et al. 2017. Sci Rep. 7:35. PubMed

Antigen Details

Antigen References

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.

Gene ID
NA

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Go To Top Version: 3    Revision Date: 01/26/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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