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FITC anti-mouse IgDa Antibody

Pricing & Availability
Clone
AMS-9.1 (See other available formats)
Other Names
Igh-5a, Igh-5.4
Isotype
Mouse (SJL) IgG2b, κ
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Product Citations
publications
AMS-9_1_FITC_070808.jpg
BALB/c mouse splenocytes stained with AMS-9.1 FITC
  • AMS-9_1_FITC_070808.jpg
    BALB/c mouse splenocytes stained with AMS-9.1 FITC
See FITC spectral data
Cat # Size Price Save
406104 500 µg ¥56,000
Description

This antibody was raised by immunization with BALB/c mouse splenocytes. It specifically reacts with mouse IgD of Igh-Ca haplotype, but does not react with b and c haplotypes. This antibody is able to induce B cell activation and proliferation.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Stall AM, et al. 1984. J. Immunol. 132:787.
  2. Goroff DK, et al. 1986. J. Immunol. 136:2382.
RRID
AB_315033 (BioLegend Cat. No. 406104)

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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