FITC anti-human TCR Vβ5 related subset Antibody

Pricing & Availability
Clone
MEM-262 (See other available formats)
Regulatory Status
RUO
Other Names
T Cell Receptor Vβ5
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
MEM-262_FITC_020708
Human peripheral blood lymphocytes stained with MEM-262 FITC
  • MEM-262_FITC_020708
    Human peripheral blood lymphocytes stained with MEM-262 FITC
  • MEM-262_FITC_111407
    Human peripheral blood lymphocytes stained with MEM-262 FITC
See FITC spectral data
Cat # Size Price Save
316704 100 tests ¥45,150
Description

The Vβ5 TCR is a variant of the TCR β chain. It belongs to Ig superfamily and is expressed by a subset of peripheral blood T cells and some T cell clones. TCR Vβ5 has been reported to recognize some staphylococcus bacterial superantigens. MEM-262 antibody reacts with a variant of the TCR β chain which is expressed on HPB-ALL cells (known expressing TCR Vβ5.3). But the subpopulation of peripheral blood T cells recognized by this antibody is reported larger than other anti-TCR Vβ5.3 antibodies.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human thymoma cell line HPB-ALL
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting, and activation of T cells Vß5 related subset.

Application References

(PubMed link indicates BioLegend citation)
  1. Pavlistova D, et al. 2003. Immunol. Lett. 88:105.
RRID
AB_528957 (BioLegend Cat. No. 316704)

Antigen Details

Structure
Ig superfamily
Distribution

T cell subset

Function
Antigen recognition, activation of TCR Vβ5 bearing T cells
Ligand/Receptor
Some staphylococcus superantigens bound to MHC
Cell Type
T cells, Tregs
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Pavlistova D, et al. 2003. Immunol. Lett. 88:105
2. Kappler T, et al. 1989. Science 244:811
3. Fernandez MM, et al. 2006. Mol. Immunol. 43:927

Gene ID
NA
UniProt
View information about TCR V beta 5 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-human TCR Vβ5 related subset

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