FITC anti-human Sialyl Lewis X (dimeric) Antibody

Pricing & Availability
Clone
FH6 (See other available formats)
Regulatory Status
RUO
Other Names
CD15s, Stage-Specific Embryonic Antigen 1, SSEA-1, Sialyl Lewis X, sLex, sLe(x), Sialyl demeric LeX, Sialyl dermic Lewis X, Sialyl Lewis X-i, sLeX-i
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
FH6_FITC_Sialyl-Lewis-X_Antibody_1_030518_updated
Human peripheral blood granulocytes were stained with sialyl lewis X (dimeric) (clone FH6) FITC (filled histogram) or mouse IgM, κ FITC isotype control (open histogram).
  • FH6_FITC_Sialyl-Lewis-X_Antibody_1_030518_updated
    Human peripheral blood granulocytes were stained with sialyl lewis X (dimeric) (clone FH6) FITC (filled histogram) or mouse IgM, κ FITC isotype control (open histogram).
  • FH6_FITC_Sialyl-Lewis-X_Antibody_2_030518_updated
    Human peripheral blood Lymphocytes were stained with sialyl lewis X (dimeric) (clone FH6) FITC and UCHT1 APC (left) or mouse IgM, κ FITC isotype control (right).
See FITC spectral data
Cat # Size Price Save
368111 25 tests ¥23,000
368112 100 tests ¥55,000
Description

The FH6 antibody recognizes Sialyl Lewis X (demeric) on glycolipids or glycoproteins. It also recognizes Sialyl Lewis X with long carbohydrate attachments (Sialyl Lewis X-i). These antigens are expressed on human granulocytes, monocytes, small subsets of lymphocytes, some fetal tissues such as the fetal stomach, fetal colon, and fetal intestine, and a variety of cancer tissues. It is believed that these antigens are involved in cell adhesion.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified 6B fucoganglioside absorbed to Salmonella minnesota.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Fukushi Y, et al. 1984. J. Biol. Chem. 259:10511.
  2. Fukushi Y, et al. 1985. Cancer Res. 8:3711.
  3. Kannagi R, et al. 1986. Cancer Research 5:2619.
  4. Kobayashi M, et al. 2010. Anticancer Res. 30:593.
RRID
AB_2728366 (BioLegend Cat. No. 368111)
AB_2728367 (BioLegend Cat. No. 368112)

Antigen Details

Structure
Carbohydrate epitope; VI3NeuAcV3III3Fuc2nLc6.
Distribution

Granulocytes, monocytes, subpopulations of lymphocytes, fetal stomach, fetal colon, fetal small intestine, and a variety of cancer tissues.

Function
Cell adhesion.
Ligand/Receptor
Selectins.
Cell Type
Granulocytes, Monocytes, Lymphocytes, Embryonic Stem Cells, Neutrophils, Neural Stem Cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Fukushi Y, et al. 1984. J. Biol. Chem. 259:10511.
2. Kannagi R, et al. 1986. Cancer Research 5:2619.
3. Nakasaki H, et al. 1989. Cancer Research 49:3662.
4. Dohi T, et al. 1993. Anticancer Res. 13:1277.

Gene ID
2526 View all products for this Gene ID
UniProt
View information about Sialyl Lewis X on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/05/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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