FITC anti-human/mouse Granzyme B Antibody

Pricing & Availability
Clone
GB11 (See other available formats)
Regulatory Status
RUO
Other Names
Granzyme-2, serine protease B, CCP1, Asp-ase, CTLA-1
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_GB11_FITC_082409
Human peripheral blood lymphocytes surface stained with CD8 PE, then intracellular stained with GB11 FITC
  • A_GB11_FITC_082409
    Human peripheral blood lymphocytes surface stained with CD8 PE, then intracellular stained with GB11 FITC
  • B_GB11_FITC_082409
    Human peripheral blood lymphocytes surface stained with CD8 PE, then intracellular stained with mouse IgG1 FITC isotype control
Compare all formats See FITC spectral data
Cat # Size Price Save
515403 25 tests ¥27,720
Description

Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Reported Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
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  6. Nacer A, et al. 2014. PLoS Pathog. 10:1004528. PubMed
  7. Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed
Product Citations
  1. Wiede F, et al. 2014. J Autoimmun. 53:105. PubMed
  2. Baker G, et al. 2014. Cancer Res. 74:5079. PubMed
  3. Sharma S, et al. 2015. J Immunol. 194:5529. PubMed
  4. Fujigaki J, et al. 2015. PLoS One. 10: 0132521. PubMed
  5. Leeansyah E, et al. 2015. PLoS Pathog. 11: 1005072. PubMed
  6. Durand J, et al. 2015. J Immunol. 195: 5035 - 5044. PubMed
  7. Bastiaens G, et al. 2016. Am J Trop Med Hyg. 94: 663 - 673. PubMed
  8. Donnarumma T, et al. 2016. Cell Rep. 17:1571-1583. PubMed
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  11. Islas-Vazquez L, et al. 2020. Biology (Basel). 9:00. PubMed
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  15. Yu M, et al. 2021. Molecular Cell. 81(6):1216-1230.e9. PubMed
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  17. Maleki KT, et al. 2021. Cell Reports Medicine. 2(3):100220. PubMed
  18. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  19. AbuEid M, et al. 2021. iScience. 24(6):102653. PubMed
  20. Zhang H, et al. 2021. Cell Reports. 35(6):109096. PubMed
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  25. Xu S, et al. 2021. Immunity. . PubMed
  26. Jeng MY, et al. 2018. J Exp Med. 215:51. PubMed
  27. Zhang J, et al. 2018. Nature. 553:91. PubMed
  28. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
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  30. Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
  31. Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
  32. Su S et al. 2018. Cell. 175(2):442-457 . PubMed
  33. Bradley T et al. 2018. Cell. 175(2):387-399 . PubMed
  34. Sagebiel AF, et al. 2019. Nat Commun. 10:975. PubMed
  35. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  36. Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed
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  40. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
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RRID
AB_2114575 (BioLegend Cat. No. 515403)

Antigen Details

Structure
32 kD serine protease
Distribution

Cytotoxic T-cells and NK cells, low on granulocytes, B cells and activated dendritic cells

Function
Induction of cell death and apoptosis
Ligand/Receptor
Mannose-6-phosphate receptor
Cell Type
B cells, Dendritic cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Enzymes and Regulators, Proteases
Antigen References

1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203
2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885
3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610
4. Wagner C, et al. 2008. Mol. Immunol. 45:1761

Gene ID
3002 View all products for this Gene ID 14939 View all products for this Gene ID 171528 View all products for this Gene ID
UniProt
View information about granzyme b on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 08/25/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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