FITC anti-human CD3 Antibody

Pricing & Availability
Clone
UCHT1 (See other available formats)
Regulatory Status
RUO
Workshop
III 471
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
UCHT1_FITC_021805
Human peripheral blood lymphocytes stained with UCHT1 FITC
  • UCHT1_FITC_021805
    Human peripheral blood lymphocytes stained with UCHT1 FITC
See FITC spectral data
Cat # Size Price Save
300405 25 tests ¥4,000
300406 100 tests ¥9,000
300452 100 µg ¥9,000
300440 500 tests ¥37,000
Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cross-Reactivity: Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
µg sizes: 0.5 mg/ml
test sizes: lot-specific (please contact technical support for concentration and total µg amount)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, and Western blotting9. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
Product Citations
  1. Tamai Y, et al. 2013. J Immunol. 190:4382. PubMed
  2. Nakatsugawa M, et al. 2015. J Immunol. 194 3487 . PubMed
  3. Ochi T, et al. 2015. Cancer Immunol Res. 3: 1070-1081. PubMed
  4. Karlsson H, et al. 2015. PLoS One. 10: 0144787. PubMed
  5. Nakatsugawa M, et al. 2016. Sci Rep. 6:23821. PubMed
  6. Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed
  7. Kariminia A, et al. 2016. Blood. 127: 3082 - 3091. PubMed
  8. Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
  9. Meermeier E, et al. 2016. Nat Commun. 7:12506. PubMed
  10. Rolandelli A, et al. 2017. Sci Rep. 7:40666. PubMed
  11. Whitfield SJC, et al. 2017. J Immunol. 198:3989. PubMed
  12. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
  13. Chen YP, et al. 2020. Cell Res. 30:1024. PubMed
  14. Jiang Y, et al. 2020. Clin Immunol. 218:108516. PubMed
  15. Vanoni G, et al. 2021. eLife. 10:00. PubMed
  16. Soday L, et al. 2021. Frontiers in Immunology. 12:600056. PubMed
  17. Cizmeci D, et al. 2021. eLife. 10:00. PubMed
  18. Klemm F, et al. 2020. Cell. 181(7):1643-1660.e17. PubMed
  19. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  20. Esaulova E, et al. 2020. Cell Host Microbe. 29(2):165-178.e8. PubMed
  21. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
  22. Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
  23. Bergamaschi L, et al. 2021. Immunity. 54(6):1257-1275.e8. PubMed
  24. Motozono C, et al. 2021. Cell Host Microbe. . PubMed
  25. Engelmann C, et al. 2017. J Transl Med. 10.1186/s12967-017-1288-3. PubMed
  26. Chapelin F, et al. 2017. Sci Rep.. 10.1038/s41598-017-17669-4. PubMed
  27. Setliff I, et al. 2018. Cell Host Microbe. 23:845. PubMed
  28. Maritz MF, et al. 2018. PLoS One. 13:e0196043. PubMed
  29. Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
  30. Barrow AD et al. 2018. Cell. 172(3):534-548 . PubMed
  31. Eri Yamada et al. 2018. Cell host & microbe. 23(1):110-120 . PubMed
  32. Johnson DC, et al. 2018. Nat Med. 24:1151. PubMed
  33. Ray RM, et al. 2019. Sci Rep. 9:4334. PubMed
  34. Chen L, et al. 2018. Immunology. 155:123. PubMed
  35. Sajadi MM, et al. 2018. Cell. 173:1783. PubMed
  36. Coufal S, et al. 2019. Cells. 8. PubMed
  37. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  38. Collins PL et al. 2018. Cell. 176(1-2):348-360 . PubMed
  39. Beyer AI, et al. 2017. Stem Cells Dev. 26:102. PubMed
  40. Corleis B, et al. 2017. PLoS One. 12:e0173161. PubMed
  41. Ryzhov S, et al. 2019. J Am Heart Assoc. 8:e010874. PubMed
  42. Wang J, et al. 2019. Oncol Lett. 4.948611111. PubMed
  43. Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed
  44. Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed
  45. Bak S, et al. 2019. Front Immunol. 9:2953. PubMed
  46. Jiang G, et al. 2020. Aging (Albany NY). 12:11466. PubMed
  47. Chang ZL, et al. 2020. Nature Protocols. 15(4):1507-1524.. PubMed
  48. Netsrithong R, et al. 2020. Stem Cell Research. 39:101487.. PubMed
  49. Huang N, et al. 2020. Genome Biol. 1.03125. PubMed
  50. Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed
  51. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  52. Bhattacharya S, et al. 2020. Front Bioeng Biotechnol. 1.055555556. PubMed
RRID
AB_314059 (BioLegend Cat. No. 300405)
AB_314060 (BioLegend Cat. No. 300406)
AB_2564148 (BioLegend Cat. No. 300452)
AB_2562046 (BioLegend Cat. No. 300440)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account