Analyte Specific Reagent. Analytical and performance characteristics are not established.
- Clone
- M-A251
- Workshop
- IV A053
- Other Names
- IL-2 receptor α chain, Low affinity IL-2R, IL-2Rα chain
- Isotype
- Mouse IgG1, κ

-
Typical results from human peripheral blood lymphocytes stained either with M-A251 FITC used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
CD25 is a 55 kD type I transmembrane glycoprotein also known as low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses. CD25 associates with IL-2 receptor β (CD122) and common γ (CD132) chains to form a high affinity IL-2R complex.
Product DetailsProduct Details
- Reactivity
- Human
- Formulation
- Phosphate-buffered solution, pH7.2, 0.09% sodium azide, 0.2% (w/v) BSA (origin USA), and a stabilizer.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
- Concentration
- 200 µg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
Suggested for Flow Cytometry
- Disclaimer
-
WARNINGS AND PRECAUTIONS
- Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
- This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
- All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
- Do not use this reagent beyond the expiration date stated on the label.
- Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be an indication of possible deterioration.
- Avoid prolonged exposure of the reagent or stained cells to light.
Antigen Details
- Antigen References
-
- Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
- Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
- Barclay N, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press Inc.
- Taniguchi T and Minami Y. 1993. Cell. 73:5-8.
- Waldmann T. 1991. J Biol Chem. 266:2681-4.
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
APC/Cyanine7 anti-human CD25
-
Purified anti-human CD25
-
PE anti-human CD25
-
FITC anti-human CD25
-
PE/Cyanine7 anti-human CD25
-
APC anti-human CD25
-
PerCP/Cyanine5.5 anti-human CD25
-
Brilliant Violet 421™ anti-human CD25
-
Alexa Fluor® 488 anti-human CD25
-
Alexa Fluor® 700 anti-human CD25
-
Brilliant Violet 510™ anti-human CD25
-
PE/Dazzle™ 594 anti-human CD25
-
Biotin anti-human CD25
-
Alexa Fluor® 647 anti-human CD25
-
Pacific Blue™ anti-human CD25
-
PerCP anti-human CD25
-
APC/Fire™ 750 anti-human CD25
-
Brilliant Violet 711™ anti-human CD25
-
Brilliant Violet 785™ anti-human CD25
-
Brilliant Violet 605™ anti-human CD25
-
KIRAVIA Blue 520™ anti-human CD25
-
PE/Fire™ 700 anti-human CD25
-
APC/Fire™ 810 anti-human CD25
-
Spark NIR™ 685 anti-human CD25 Antibody
-
Spark YG™ 581 anti-human CD25
-
PE/Fire™ 640 anti-human CD25 Antibody
-
PE anti-human CD25
-
PerCP/Cyanine5.5 anti-human CD25
-
APC/Fire™ 750 anti-human CD25
-
PE/Cyanine7 anti-human CD25
-
APC anti-human CD25
-
PE/Cyanine5 anti-human CD25
-
FITC anti-human CD25
-
Spark Red™ 718 anti-human CD25
-
GMP PE anti-human CD25
-
GMP PE/Cyanine7 anti-human CD25
-
PerCP/Fire™ 780 anti-human CD25
Follow Us