Cyanine5 Streptavidin

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Other Names
SAv-Cyanine5
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Product Citations
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FrancescaMazzoni.jpg
Unpassaged live mouse primary retinal pigment epithelial cells engulfed biotinylated photocreceptor outer segments (POS) coupled to Cyanine5 Streptavidin (red). Cells were subsequently stained with Flash Phalloidin™ Green 488 (green) and DAPI (blue). Image generously submitted to the 2017 Cell Life Imaging Competition by Francesca Mazzoni from Fordham University.
  • FrancescaMazzoni.jpg
    Unpassaged live mouse primary retinal pigment epithelial cells engulfed biotinylated photocreceptor outer segments (POS) coupled to Cyanine5 Streptavidin (red). Cells were subsequently stained with Flash Phalloidin™ Green 488 (green) and DAPI (blue). Image generously submitted to the 2017 Cell Life Imaging Competition by Francesca Mazzoni from Fordham University.
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Cat # Size Price Save
405209 100 µg ¥35,000
Description

Streptavidin binds to biotin with high affinity. Streptavidin-Cy5 is useful for detecting biotinylated antibodies. The excitation of Cy5 by 625-650 nm laser light induces a fluorescence emission maximum of 670 nm.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with Cyanine5 under optimal conditions.
Concentration
0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The streptavidin-Cy5 solution should be stored undiluted between 2°C and 8°C, and protected from exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested

Recommended Usage

Each lot of this Streptavidin-Cyanine5 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Cyanine5 conjugation. For immunofluorescent staining, the recommended use of this reagent is = 0.5 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Streptavidin-Cy5 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of streptavidin-Cy5 is 60 kD and Streptavidin alone is 52 kD.

Additional Product Notes

BioLegend is in the process of converting the name Cy5 to Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our Cyanine5 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Hsu HC, et al. 2007. J. Immunol. 178:5357.
  2. Biegler KA, et al. 2012. Cancer Prev Res (Phila). 5:1173. PubMed
  3. Cheng JY, et al. 2014. Cancer Res. 74:6856. PubMed
Product Citations
  1. Biegler K, et al. 2012. Cancer Prev Res. 5:1173. PubMed
  2. Cheng J, et al. 2014. Cancer Res. 74:6856. PubMed
  3. Dedeoglu B, et al. 2016. PLoS One. 11: 0150826. PubMed
  4. Müller-Durovic B, et al. 2016. J Immunol. 197: 2891 - 2899. PubMed

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Go To Top Version: 4    Revision Date: 06/18/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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