Brilliant Violet 750™ anti-human CD8 Antibody, CD8a, SK1

Clone: SK1 (See other available formats)
Regulatory Status: RUO
Other Names: T8, Leu2
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human peripheral blood lymphocytes were stained with CD8 (clone SK1) Brilliant Violet 750™ and CD3 FITC (left) or with CD3 FITC only (right).

Compare all formats See Brilliant Violet 750™ spectral data

Cat #	Size	Price	Save
344755	25 tests	¥42,840
344756	100 tests	¥87,780

Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α₃ domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details

Technical data sheet

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Chimpanzee, Pigtailed Macaque, Sooty Mangabey

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 750™ under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 750™ excites at 405 nm and emits at 750 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 750™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics⁸, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)^9,10. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.

Application References (PubMed link indicates BioLegend citation)

Ledbetter JA, et al. 1981. J. Exp. Med. 153:310.
Campanelli R, et al. 2002. Intl. Immunol. 14:39.
Evans RL, et al. 1981. Immunol. 78:544.
Wooldridge L, et al. 2005. J. Bio. Chem. 280:27491.
Ch'el IL, et al. 2011. J Exp Med. 208:633. PubMed
Carbone A, et al. 1999. Blood 93:2319. (IHC-F)
Ahmed A, et al. 2001. J. Pathol. 193:383. (IHC)
Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

RRID

AB_2810546 (BioLegend Cat. No. 344755)
AB_2810547 (BioLegend Cat. No. 344756)

Antigen Details

Structure: Ig superfamily, homodimer or heterodimer with CD8b, 32-34 kD
Distribution: Majority of thymocytes, T cell subset, NK cells
Function: MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor: MHC Class I molecules
Cell Type: NK cells, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
Gene ID: 925 View all products for this Gene ID
UniProt: View information about CD8 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

There are no FAQs for this product.

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KIRAVIA Blue 520™ anti-human CD8	SK1	FC
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Concentration & Expiration Lookup

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 06/03/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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