Brilliant Violet 510™ anti-human CD14 Antibody

Pricing & Availability
Clone
M5E2 (See other available formats)
Regulatory Status
RUO
Workshop
III 329
Other Names
LPS receptor
Isotype
Mouse IgG2a, κ
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Product Citations
publications
M5E2_BV510_CD14_Antibody_FC_082312
Human peripheral blood monocytes were stained with CD14 (clone M5E2) Brilliant Violet 510™.
  • M5E2_BV510_CD14_Antibody_FC_082312
    Human peripheral blood monocytes were stained with CD14 (clone M5E2) Brilliant Violet 510™.
See Brilliant Violet 510™ spectral data
Cat # Size Price Save
301841 25 tests ¥37,000
301842 100 tests ¥73,000
Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Capuchin Monkey, Cattle (Bovine, Cow), Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Cynomolgus, Dog (Canine), Rhesus, Pigtailed Macaque, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length human CD14 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).

Application References

(PubMed link indicates BioLegend citation)
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F)
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  4. Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
  5. Williams KC, et al. 2001. J. Exp. Med. 193:905.
  6. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
  7. Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Zizzo G, et al. 2012. J. Immunol. 189:3508. PubMed
  10. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  11. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Rutishauser L, et al. 2017. AIDS Res Hum Retroviruses . 10.1089/AID.2016.0324. PubMed
  2. Martrus G, et al. 2017. PLoS One.. 10.1371/journal.pone.0182532. PubMed
  3. Garfall AL, et al. 2018. JCI Insight. 3. PubMed
  4. Bradley T et al. 2018. Cell. 175(2):387-399 . PubMed
  5. Liu Y, et al. 2019. Nat Commun. 10:324. PubMed
  6. Sagebiel AF, et al. 2019. Nat Commun. 10:975. PubMed
  7. Baxter AE, et al. 2017. Nat Protoc. 12:2029. PubMed
  8. Cassetta L et al. 2019. Cancer Cell. 35(4):588-602 . PubMed
  9. Gomez–Ospina N, et al. 2019. Nat Commun. 3.225694444. PubMed
  10. Abd Hamid M et al. 2019. Cancer Immunol Res. 7(8):1293-1306 . PubMed
  11. Andrews SF et al. 2019. Immunity. 51(2):398-410 . PubMed
  12. Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed
  13. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  14. Moguche AO et al. 2017. Cell host & microbe. 21(6):695-706 . PubMed
  15. Escudero–Pérez B, et al. 2019. JCI Insight. 4:e126070. PubMed
  16. Jegaskanda S, et al. 2019. JCI Insight. 4:e129035. PubMed
  17. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  18. Giron LB, et al. 2020. EBioMedicine. 73.94791667. PubMed
  19. Morris AB, et al. 2020. Immunity. 52(1):136-150.e6.. PubMed
  20. Sekine T, et al. 2020. Cell. 183:158. PubMed
  21. Colby DJ, et al. 2018. Nat Med. 24:923. PubMed
  22. Martins M, et al. 2015. J Virol. 89: 10802 - 10820. PubMed
  23. Glaser K, et al. 2016. PLoS One. 11: 0146898. PubMed
  24. Wheatley A, et al. 2016. Sci Rep. 6: 26478. PubMed
  25. Castro I, et al. 2016. J Virol. 90: 5280 - 5291. PubMed
  26. Demers K, et al. 2016. PLoS Pathog. 12: 1005805. PubMed
  27. Woda M, et al. 2016. J Infect Dis. 214: 1001 - 1009. PubMed
  28. Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed
  29. Rakshit S, et al. 2020. Cell Rep. 33:108451. PubMed
  30. Juno JA, et al. 2020. Nat Med. 26:1428. PubMed
  31. Scharenberg SG, et al. 2020. Nat Commun. 2.76875. PubMed
  32. Asokan M, et al. 2020. Proc Natl Acad Sci U S A. 117:18754. PubMed
  33. Eslamizar L, et al. 2021. NPJ Vaccines. 6:15. PubMed
  34. Luoma AM, et al. 2020. Cell. 182(3):655-671.e22. PubMed
  35. Buggert M, et al. 2020. Cell. 183(7):1946-1961.e15. PubMed
  36. Nielsen CM, et al. 2021. Cell Reports Medicine. 2(3):100207. PubMed
  37. Sutton HJ, et al. 2021. Cell Reports. 34(6):108684. PubMed
  38. Townsley SM, et al. 2021. Cell Host Microbe. 29(4):564-578.e9. PubMed
  39. Mukhamedova M, et al. 2021. Immunity. 54(4):769-780.e6. PubMed
  40. Graham C, et al. 2021. Immunity. 54(6):1276-1289.e6. PubMed
  41. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
RRID
AB_2561379 (BioLegend Cat. No. 301841)
AB_2561946 (BioLegend Cat. No. 301842)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 11/05/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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