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Brilliant Violet 421™ anti-human CD36 Antibody

Pricing & Availability
Clone
5-271 (See other available formats)
Regulatory Status
RUO
Other Names
GPIIIb, GPIV
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
5-271_BV421_CD36_Antibody_080519
Human peripheral blood platelets were stained with CD36 (clone 5-271) Brilliant Violet 421™ (filled histogram) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
  • 5-271_BV421_CD36_Antibody_080519
    Human peripheral blood platelets were stained with CD36 (clone 5-271) Brilliant Violet 421™ (filled histogram) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Save
336229 25 tests ¥41,140
336230 100 tests ¥91,960
Description

CD36 is an 85 kD integral membrane glycoprotein, also known as GPIIIb, or GPIV. It is expressed on various epithelial and endothelial cells as well as erythrocytes, platelets, macrophages/monocytes and some macrophage-derived dendritic cells. CD36 functions as a scavenger receptor, binding thrombospondin, long chain fatty acids, oxidized LDL, collagen type I, IV, and V as well as apoptotic cells. The 5-271 antibody has been reported to be useful for flow cytometry.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human platelets
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence2.

Application References

(PubMed link indicates BioLegend citation)
  1. Stelner E, et al. 2006. J. Cell Sci. 119:459.
  2. Stewart DA, et al. 2012. Mol. Cancer Res. 10:727. (IF)
Product Citations
  1. Ma X, et al. 2021. Cell Metabolism. 33(5):1001-1012.e5. PubMed
  2. Xu S, et al. 2021. Immunity. . PubMed
RRID
AB_2814227 (BioLegend Cat. No. 336229)
AB_2814228 (BioLegend Cat. No. 336230)

Antigen Details

Structure
Integral membrane protein, CD36 family, single chain glycoprotein, 85-113 kD
Distribution

Expressed in various epithelial and endothelial cells, erythrocytes, platelets, monocytes/ macrophages, some macrophage-derived dendritic cells

Function
Scavenger receptor for thrombospondin, long chain fatty acids, anionic phospholipids, oxidized LDL, collagen types I, IV and V. Involved in recognition and phagocytosis of apoptotic cells, cell adhesion.
Ligand/Receptor
Thrombospondin, anionic phospholipids, oxidized LDL, collage types I, IV, and V
Cell Type
Dendritic cells, Endothelial cells, Epithelial cells, Erythrocytes, Macrophages, Monocytes, Platelets
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Hogg N, et al. 1984. Immunology 53-753.
2. Greenwalt DE, et al. 1992. Blood 80:1105.
3. Armsesilla AL, et al.1994. J. Biol. Chem. 269:18985.
4. Endemann G, et al. 1993. J. Biol. Chem. 268:11811.

Gene ID
948 View all products for this Gene ID
UniProt
View information about CD36 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 08/05/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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