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Brilliant Violet 421™ anti-human CD244 (2B4) Antibody

Pricing & Availability
Clone
2-69 (See other available formats)
Regulatory Status
RUO
Other Names
2B4, NAIL, SLAMF4
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
2-69_BV421_CD244_Antibody_060818
Human lysed whole blood was stained with CD56 PerCP/Cy5.5 and CD244 (2B4, clone 2-69) Brilliant Violet™ 421 (left) or Mouse IgG2a, κ Brilliant Violet™ 421 isotype control (right).
  • 2-69_BV421_CD244_Antibody_060818
    Human lysed whole blood was stained with CD56 PerCP/Cy5.5 and CD244 (2B4, clone 2-69) Brilliant Violet™ 421 (left) or Mouse IgG2a, κ Brilliant Violet™ 421 isotype control (right).
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Save
393503 25 tests ¥44,220
393504 100 tests ¥83,820
Description

CD244, known as 2B4, is a 38 kD type I transmembrane protein. It is a member of the CD2 subset of the immunoglobulin superfamily (IgSF) molecules. CD244 is expressed on NK cells, a subset of T cells (including most CD8+ T cells and γ/δ T cells), monocytes, basophils, and eosinophils. CD48 is the ligand of CD244. It has been reported that ligation of human CD244 results in enhanced NK cell cytotoxicity and cytokine production. Recent studies have shown that human CD244, like murine CD244, has both activating and inhibitory functions, which are dependent on the density of surface 2B4 expression, degree of ligation, and the level of the adaptor molecule SAP expression.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human NK cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: redirected killing assays (rADCC) in NK cells1.

Application References

(PubMed link indicates BioLegend citation)
  1. Nakajima H, et al. 2000. Eur J Immunol. 30:3309. (Cytotoxicity, IP)
  2. Tassi I, Colonna M. 2005. J. Immunol. 7996-8002. (IP)
RRID
AB_2734461 (BioLegend Cat. No. 393503)
AB_2734462 (BioLegend Cat. No. 393504)

Antigen Details

Structure
38 kD type I transmembrane protein, CD2 family member, Ig superfamily
Distribution

NK, T subset, monocytes, basophils, and eosinophils

Function
Activation and inhibition of NK cell function
Ligand/Receptor
CD48
Cell Type
Basophils, Eosinophils, Monocytes, NK cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Lee KM, et al. 2003. J. Immunol. 170:4881.
  2. Chlewicki LK, et al. 2008. J. Immunol. 180:8159.
  3. Messmer B, et al. 2006. J. Immunol. 176:4646.
  4. Boles KS, et al. 2001. Immunol. Rev. 181:234.
Gene ID
51744 View all products for this Gene ID
UniProt
View information about CD244 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 06/08/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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