Brilliant Violet 421™ anti-CD105 (Endoglin) Antibody

Pricing & Availability
Clone
SN6h (See other available formats)
Regulatory Status
RUO
Other Names
Endoglin
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SN6h_BV421_CD105_Endoglin_Antibody_041917
Human monocytic cell line THP-1 was stained with CD105 (clone SN6h) Brilliant Violet™ 421 (filled histogram) or mouse IgG1, κ Brilliant Violet™ 421 isotype control (open histogram).
  • SN6h_BV421_CD105_Endoglin_Antibody_041917
    Human monocytic cell line THP-1 was stained with CD105 (clone SN6h) Brilliant Violet™ 421 (filled histogram) or mouse IgG1, κ Brilliant Violet™ 421 isotype control (open histogram).
See Brilliant Violet 421™ spectral data
Cat # Size Price Save
800509 25 tests ¥35,000
800510 100 tests ¥76,000
Description

Endoglin (CD105) is a type I membrane glycoprotein that is part of the TGF beta receptor complex. It is expressed in vascular endothelial tissue as well as a variety of other tissues. Endoglin may also be involved in the cytoskeletal organization affecting cell morphology and migration.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CD105 (Endoglin) isolated from acute lymphoblastic leukemia cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. C Li, R Grady, BK Seon, SE Duff, S Abdalla, A Renehan, ST O'Dwyer, N Haboubi, S Kumar. Both high intratumoral microvessel density determined using CD105 antibody and elevated plasma levels of CD105 antibody in colorectal cancer patients correlate with poor prognosis. Br J Cancer 88:1424-1431, 2003.
  2. Sanz-Rodriguez, Francisco, et al. Endoglin regulates cytoskeletal organization through binding to ZRP-1 a member of the Lim family of proteins. J Biol Chem 279(31):32858-68, 2004.
  3. Takahashi N, et al. Association of Serum Endoglin with Metastasis in Patients with Colorectal, Breast, and Other Solid Tumors, and SuppressiveEffect of Chemotherapy on the Serum Endoglin. Clin. Cancer Res.: 7(3): 524-532, Mar 2001.
  4. Seon BK, Matsuno F, Haruta Y, Kondo M, and Barcos M. Long-lasting complete inhibition of human solid tumors in SCID mice by targeting endothelial cells of tumor vasculature with antihuman endoglin immunotoxin. Clin. Cancer Res.: 3(7): 1031-1044, Jul 1997.
  5. Haruta Y, Seon BK. Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6. PNAS: 83: 7898-7902, Oct 1986.
RRID
AB_2687062 (BioLegend Cat. No. 800509)
AB_2687063 (BioLegend Cat. No. 800510)

Antigen Details

Cell Type
Mesenchymal Stem Cells
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
CD Molecules
Gene ID
2022 View all products for this Gene ID
UniProt
View information about CD105 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 0    Revision Date: 04/19/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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