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Biotin anti-Tau, 419-433 Antibody

Pricing & Availability
Clone
A16097D (See other available formats)
Regulatory Status
RUO
Other Names
Microtubule associated protein tau
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A16097D_Biotin_Tau419-433_Antibody_1_092418
Western blot of Biotin anti-Tau, 419-433 antibody (clone A16097D). Lane 1: Molecular weight marker; Lane 2: 20 µg of normal human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 0.20 µg/mL of clone A16097D or Rat IgG2a, κ Isotype Ctrl antibody (Cat. No. 400502) overnight at 4°C. The blot that was incubated with clone A16097D was probed with HRP Streptavidin (Cat. No. 405210). The blot that was incubated with Rat IgG was probed with HRP Goat anti-rat IgG secondary antibody (Cat. No. 405405). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • A16097D_Biotin_Tau419-433_Antibody_1_092418
    Western blot of Biotin anti-Tau, 419-433 antibody (clone A16097D). Lane 1: Molecular weight marker; Lane 2: 20 µg of normal human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 0.20 µg/mL of clone A16097D or Rat IgG2a, κ Isotype Ctrl antibody (Cat. No. 400502) overnight at 4°C. The blot that was incubated with clone A16097D was probed with HRP Streptavidin (Cat. No. 405210). The blot that was incubated with Rat IgG was probed with HRP Goat anti-rat IgG secondary antibody (Cat. No. 405405). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • A16097D_Biotin_Tau419-433_Antibody_2_092418
    IHC staining of Biotin anti-Tau, 419-433 antibody (clone A16097D) on formalin-fixed paraffin-embedded normal human (left panel) and Alzheimer’s disease (right panel) brain tissues. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissues were incubated with 1 µg/mL of the primary antibody overnight at 4°C. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit was used (Multi-Species, component #5, DAB; Cat. No. 929901). The slides were counterstained with hematoxylin, according to the protocol provided. The images were captured with a 40X objective.
Compare all formats
Cat # Size Price Save
851005 25 µg ¥26,670
Description

Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyper-phosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain.  Tau isoforms are differentially expressed during development.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant C-terminal fragment of human 4R Tau protein encompassing amino acid residues 419-433.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.2 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 0.2 - 1.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody cross-reacts with 3R and 4R Tau isoforms, and recognizes endogenous human and mouse Tau in brain lysates by WB. 

RRID
AB_2783420 (BioLegend Cat. No. 851005)

Antigen Details

Structure
Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
Distribution

Tissue distribution: central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: cytoskeleton, nucleus, plasma membrane and cytosol.

Function
Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Interaction
Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Tau
Antigen References
  1. Augustinack JC, et al. 2002. Acta Neuropathol. 103(1):26-35. PubMed.
  2. Seubert P, et al. 1995. J Biol Chem. 270(32):18917-22.PubMed.
  3. Dong Y, et al. 2012. PLoS One 7(6):e39386. PubMed.
Gene ID
4137 View all products for this Gene ID
UniProt
View information about Tau, 419-433 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All Tau, 419-433 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-Tau, 419-433 A16097D WB,Direct ELISA,IHC-P
HRP anti-Tau, 419-433 A16097D WB,IHC-P
Biotin anti-Tau, 419-433 A16097D WB,IHC-P
Go To Top Version: 2    Revision Date: 10/11/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-Tau, 419-433

  • HRP anti-Tau, 419-433

  • Biotin anti-Tau, 419-433
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