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Biotin anti-mouse Lineage Panel

Pricing & Availability
Clone
145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70;
Regulatory Status
RUO
Other Names
Lin-1
Isotype
Armenian Hamster IgG/Rat IgG2a/Rat IgG2b
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Product Citations
publications
MU_Biotin_Lineage_Cocktail_012712
C57BL/6 mouse bone marrow cells were stained with Biotin Lineage Cocktail, followed by Sav-FITC and CD117 APC.
  • MU_Biotin_Lineage_Cocktail_012712
    C57BL/6 mouse bone marrow cells were stained with Biotin Lineage Cocktail, followed by Sav-FITC and CD117 APC.
Compare all formats
Cat # Size Price Save
133307 500 tests ¥63,630
Description

The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The Biotin Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3ε, clone 145-2C11; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER-119/Erythroid cells, clone Ter-119. Biotin Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative control immunoglobulin.

Materials provided:
1. Biotin anti-mCD3ε/ Biotin anti-mGr-1/ Biotin anti-mCD11b/ Biotin anti-mCD45R(B220)/ Biotin anti-mTer-119, 25 tests
2. Biotin Armenian hamster IgG/ Biotin Rat IgG2b/ Biotin Rat IgG2a isotype controls, 25 tests


Materials not provided:
Cell Staining Buffer (Cat. No. 420201)

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solutions should be stored undiluted at 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Magnetic particle separation - Verified
Recommended Usage

Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 2 µl of each antibody per million cells or 2 µl of each antibody per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Morris SJ, et al. 1997. Development. 124(10):1929-1939
  2. Okada S, et al. 1992. Blood180 (12):3044-3050
  3. Spangrude GJ, et al. 1988. Science 241 (4861):58-62
  4. Spangrude GJ, et al. 1990. Exp Hematol. 18 (8):920-926
  5. Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed
  6. Ito R, et al. 2012. Exp Hematol. 40:953. PubMed.
Product Citations
  1. Shen Y, et al. 2021. Comput Struct Biotechnol J. 19:5360. PubMed
  2. Bonnet C, et al. 2021. iScience. 24:103399. PubMed
  3. LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed
  4. Grizotte–Lake M, et al. 2018. Immunity. 49:1103. PubMed
  5. Ni F, et al. 2019. Cell Stem Cell. 24:608. PubMed
  6. Carpenter RS, et al. 2020. Nat Commun. 3.029166667. PubMed
  7. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  8. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  9. Breitbach M, et al. 2018. Cell Stem Cell. 1.098611111. PubMed
  10. de Almeida MJ et al. 2017. Cell stem cell. 21(6):725-729 . PubMed
  11. Zhang Z, et al. 2019. Haematologica. 4.339583333. PubMed
  12. Freter R, et al. 2021. Nat Commun. 12:3318. PubMed
  13. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  14. Morimoto Y, et al. 2018. Immunity. 49:134. PubMed
  15. Ha TC, et al. 2020. Hum Gene Ther. 32:458. PubMed
  16. Keerthivasan S, et al. 2021. Immunity. 54(7):1511-1526.e8. PubMed
  17. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  18. Fujimoto M, et al. 2022. Nat Commun. 13:5408. PubMed
  19. Guo L, et al. 2020. Nat Commun. 0.972222222. PubMed
  20. Li X, et al. 2019. Stem Cells. 37:937. PubMed
  21. Lion M, et al. 2020. Proc Natl Acad Sci U S A. 117:5453. PubMed
RRID
AB_11124348 (BioLegend Cat. No. 133307)

Antigen Details

Distribution

T cells, B cells, monocytes/macrophages, granulocytes, NK cells, and erythrocytes

Biology Area
Immunology
Antigen References

1. Morrison SJ, et al. 1997. Development 124:1929.
2. Okada S, et al. 1992. Blood 80:3044.
3. Spangrude GJ, et al. 1988. Science 241:58.
4. Spangrude GJ, et al. 1990. Exp Hematol. 18:920.
5. Tan SL, et al. 2006. J. Immunol. 176:2872.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about Lineage on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All Lineage Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse Lineage Cocktail with Isotype Ctrl 145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70 FC
PE anti-mouse Lineage Cocktail with Isotype Ctrl 145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70 FC
Biotin anti-mouse Lineage Panel 145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70 FC
Go To Top Version: 2    Revision Date: 10/23/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-mouse Lineage Cocktail with Isotype Ctrl

  • PE anti-mouse Lineage Cocktail with Isotype Ctrl

  • Biotin anti-mouse Lineage Panel
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