Biotin anti-mouse IgEa Antibody

Pricing & Availability
UH297 (See other available formats)
Regulatory Status
Other Names
1. Usui, M.
Rat IgG1, κ
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Product Citations
Cat # Size Price Save
408804 500 µg ¥49,770

The UH297 antibody reacts specifically with mouse soluble immunoglobulin E of Igh-Ca haplotype (e.g., A, AKR, BALB/c, C3H/He, CBA). It does not react with IgE Igh-cb haplotype (e.g., C57BL/6, C57BL/10, SJL) and other Ig isotypes. UH297 mAb can be used for immunofluorescent staining of cell surface IgEa and ELISA applications.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
BALB/c mouse IgE anti-DNP mAb from DNPε 117 hybridoma
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

ELISA - Quality tested
FC, WB - Reported in the literature, not verified in house

Recommended Usage
Each lot of this antibody is quality control tested by ELISA. For ELISA detection, the suggested use of this reagent is 1-2 µg per ml. It's recommended that the reagent be titrated for optimal performance for each application.
Application References

(PubMed link indicates BioLegend citation)
  1. Usui M, et al. 1993. Immunogenetics. 37:301-304
  2. Yamada M, et al. 1994. Immunogenetics. 40:296-299
Product Citations
  1. Cárdenas–Torres FI, et al. 2019. Medicina (Kaunas). 55:3. PubMed
AB_1937222 (BioLegend Cat. No. 408804)

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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  • Biotin anti-mouse IgEa


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