Biotin anti-human IL-34 Antibody

Pricing & Availability
Clone
E033B8 (See other available formats)
Other Names
Interleukin 34, C16orf77
Isotype
Mouse IgG2a, λ
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Product Citations
publications
Cat # Size Price Save
361401 50 µg ¥30,000
361402 500 µg ¥85,000
Description

Human IL-34 shares a sequence identity of 99.6%, 72% and 71% with chimpanzee, rat, and mouse IL-34, respectively, on the amino acid level. The IL-34 gene is syntenic in the human, chimpanzee, rat, and mouse genomes. IL-34 has no apparent consensus structural domain or motif, and does not share sequence homology with M-CSF; nevertheless, it binds to the CSFR. These two cytokines are not identical in biological activity and signal activation. IL-34 and CSF show an equivalent ability to support cell growth or survival. However, these cytokines are differing in their ability to induce the production of chemokines (MCP-1 and eotaxin-2) in primary macrophages, the morphological change in TF-1-fms cells, and the migration of J774A.1 cells. The use of monoclonal antibodies against the CSFR suggests differential domain binding of the receptor to IL-34 and CSF and as a result, different bioactivities and signal activation kinetics/strength are produced for these cytokines.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human IL-34
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.5 - 2.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of human IL-34 recombinant protein ranging from 5000 to 78 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: The biotinylated E033E8 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified E0320E7 antibody (Cat. No. 361301) as the capture antibody and recombinant human IL-34 (Cat. No. 577909) as the standard.

Note: For testing human IL-34 in serum, plasma or cell culture supernatant, LEGEND MAX™ Human IL-34 ELISA Kit with Pre-coated Plates (Cat. No. 439607 & 439608) are specially developed and recommended.

RRID
AB_2563034 (BioLegend Cat. No. 361401)
AB_2563035 (BioLegend Cat. No. 361402)

Antigen Details

Structure
Secreted glycoprotein, homodimer
Distribution

IL-34 mRNA is expressed in many different tissues including: spleen, heart, brain, lung, liver, kidney, thymus, testes, ovary, small intestine, prostate, and colon

Function
IL-34 increases monocyte viability, induces macrophage proliferation, and synergizes with other cytokines to generate macrophages and osteoclasts from cultured progenitors. IL-34 promotes the formation of the colony forming unit-macrophage (CFU-M) in human bone marrow culture. IL-34 supports RANKL-induced osteoclastogenesis by promoting the adhesion and proliferation of osteoclast progenitors. Human IL-34 promotes osteoclast differentiation from peripheral blood mononucleated cells. In addition, systematic administration of IL-34 to mouse increases the number of CD11b+ cells and reduces bone mass. IL-1ß and TNF-α induce the expression of interleukin-34 mRNA in osteoblasts. IL-34 is also induced in macrophages infected with equine infectious anemia virus (EIAV).
Interaction
Macrophages, monocytes, myeloid cells
Ligand/Receptor
CSF-1R
Biology Area
Cell Biology, Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Clanchy FI, et al. 2006. J. Leuko. Biol. 79:757. 
2. Lin H, et al. 2008. Science 320:807. 
3. Chihara T, et al. 2010. Cell. Death Differ. 17:1917. 
4. Wei S, et al. 2010. J. Leuko. Biol. 88:495. 
5. Eda H, et al. 2011. Rheumatol. Int. 31:1525.

Gene ID
146433 View all products for this Gene ID
UniProt
View information about IL-34 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 01/21/2019

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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