Biotin anti-human CD81 (TAPA-1) Antibody

Pricing & Availability
Clone
5A6 (See other available formats)
Other Names
S5.7, CVID6, TSPAN28
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
5A6_Biotin_CD81_Antibody_031816
Human peripheral blood lymphocytes were stained with biotinylated anti-human CD81 (clone 5A6) (filled histogram) or biotinylated mouse IgG1, κ (open histogram), followed by SAV-PE
  • 5A6_Biotin_CD81_Antibody_031816
    Human peripheral blood lymphocytes were stained with biotinylated anti-human CD81 (clone 5A6) (filled histogram) or biotinylated mouse IgG1, κ (open histogram), followed by SAV-PE
Cat # Size Price Save
349514 100 µg ¥31,000
Description

CD81 is a 26 kD non-glycosylated member of the tetraspanin superfamily (TM4SF), also known as TAPA-1 (target of an antiproliferative antibody). CD81 is expressed on T and B cells, NK cells, monocytes, dendritic cells, thymocytes, endothelial cells, and fibroblasts. It also has low levels of expression on granulocytes. CD81 induces B cell adhesion via VLA-4 integrin and has been shown to play a role in early T cell development. CD81 associates with several other cell-surface proteins in a multimolecular complex, including CD19, CD21, CD20, CD37, CD53, and CD82 in B cells, and CD4, CD8, and CD82 in T cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human OCI-LY8 cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western Blotting3 and immunoprecipitation2,3.

Application References

(PubMed link indicates BioLegend citation)
  1. Menno C, et al. 2010. J. Clin. Invest. 4:1265.
  2. Oren R, et al. 1990. Mol. Cell. Biol. 8:4007. (IP)
  3. Clark K, et al. 2004. J. Biol. Chem. 279(19):19401. (IP, WB)
  4. Mochida K, et al. 2008. J. Virol. 13:6711.
  5. Rappa G, et al. 2014. Mol Cancer Res. 12:1840. PubMed
RRID
AB_2572038 (BioLegend Cat. No. 349514)

Antigen Details

Structure
26 kD, type III transmembrane protein, member of the TM4SF tetraspanin family. Complexed with CD82, CD19, CD21, or CD4, CD8.
Distribution

T cells, NK, monocytes, B cells, endothelial and epithelial cells, low on granulocytes.

Function
Regulates cell activation and growth and cell aggregation.
Cell Type
B cells, Endothelial cells, Epithelial cells, Monocytes, Neural Stem Cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Transcription Factors
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Menno C, et al. 2010. J. Clin. Invest. 4:1265.
2. Fearon D, et al. 1995. Annu. Rev. Immunol. 13:127.
3. Wright M, et al. 1994. Immunol. Today 15:588.

Gene ID
975 View all products for this Gene ID
UniProt
View information about CD81 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 03/22/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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