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- Clone
- O92E4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PECAM-1, EndoCAM
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CD31 is a 130-140 kD type I transmembrane glycoprotein also known as platelet endothelial cell adhesion molecule-1 (PECAM-1) or Endocam. It is expressed on monocytes, platelets, granulocytes, endothelial cells and lymphocyte subsets. CD31 has been reported to bind CD38 and be involved in wound healing, angiogenesis, and cellular migration in response to inflammation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full length human recombinant protein of human PECAM1 produced in HEK293T cell.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ELISA Detection - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For ELISA Detection applications, a concentration range of 0.25 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of CD31 recombinant protein ranging from 10 to 0.156 ng/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The biotinylated O92E4 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified WM59 (Cat. No. 303102) antibody as the capture antibody.
Note: For testing human CD31 in cell culture supernatant, serum, plasma, other biological fluids, BioLegend's LEGEND MAX™ Kits (Cat. No. 443607 & 443608) are specially developed and recommended. - RRID
-
AB_2783341 (BioLegend Cat. No. 536604)
Antigen Details
- Structure
- Ig superfamily, type I transmembrane glycoprotein, 130-140 kD
- Distribution
-
Monocytes, platelets, granulocytes, endothelial cells, lymphocyte subset
- Function
- Cell adhesion, signal transduction
- Ligand/Receptor
- CD38
- Cell Type
- Endothelial cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, Platelets
- Biology Area
- Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Antigen References
-
- DeLisser H, et al. 1994. Immunol. Today 15:490.
- Newman P, 1997. J. Clin. Invest. 99:3.
- Fawcett J, et al. 1995. J. Cell Biol. 128:1229.
- Gene ID
- 5175 View all products for this Gene ID
- UniProt
- View information about CD31 on UniProt.org
Related FAQs
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Other Formats
View All CD31 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Biotin anti-human CD31 | O92E4 | ELISA Detection |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Biotin anti-human CD31
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