Biotin anti-CD107b (LAMP-2) Antibody

Pricing & Availability
Clone
ABL-93 (See other available formats)
Regulatory Status
RUO
Other Names
Lysosome-associated membrane glycoprotein 2, lysosomal membrane glycoprotein 2, CD107 antigen-like family member B, lysosome-associated membrane protein 2
Isotype
Rat IgG2a
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Product Citations
publications
ABL-93_Biotin_CD107b_Antibody_040519.png
IHC staining of Biotin anti-CD107b (LAMP2) antibody (clone ABL-93) on formalin-fixed paraffin-embedded mouse choroid plexus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 streptavidin (Cat. No. 405237) for one hour at room temperature. The image was captured with a 40X objective. Scale bar: 50 µm
  • ABL-93_Biotin_CD107b_Antibody_040519.png
    IHC staining of Biotin anti-CD107b (LAMP2) antibody (clone ABL-93) on formalin-fixed paraffin-embedded mouse choroid plexus tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 streptavidin (Cat. No. 405237) for one hour at room temperature. The image was captured with a 40X objective. Scale bar: 50 µm
  • ABL-93_Biotin_CD107b_Antibody_040519.png
    IHC staining of Biotin anti-CD107b (LAMP2) antibody (clone ABL-93) on formalin-fixed paraffin-embedded mouse kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 streptavidin (Cat. No. 405237) for one hour at room temperature. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Save
816105 25 µg ¥22,000
816106 100 µg ¥51,000
Description

CD107b, also known as LAMP-2, is a 105 kD, highly glycosylated, type I transmembrane protein. CD107b is expressed in lysosomal/endosomal membranes in nearly all cells, on the surface of activated platelets, activated lymphocytes and some tumor cell lines.  LAMP-2 is known to have roles in cell adhesion and cellular homeostasis, including autophagocytosis and antigen presentation.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
This monoclonal antibody was raised against Balb/c 3T3 mouse embryo fobroblast tissue culture cell glycoproteins. It has been mapped to the N-terminus of LAMP 2.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC-P).

This antibody is specific to mouse LAMP-2. LAMP-2 molecular weight appears to be at ~105 kDa on the gel due to high glycosylation.

RRID
AB_2801146 (BioLegend Cat. No. 816105)
AB_2801147 (BioLegend Cat. No. 816106)

Antigen Details

Structure
LAMP-2 is a 415 amino acid protein with a molecular mass of 45 kD.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
CD Molecules
Gene ID
16784 View all products for this Gene ID
UniProt
View information about CD107b on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 04/05/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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