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The Aβ Antibody Sampler Kit offers flexibility for sampling and detection of full-length amyloid precursor protein (APP) and various APP cleavage fragments including Aβ 1-40 and Aβ 1-42 peptides. The kit also includes clone 3A1, which offers unique advantages over gold standard antibodies for Aβ detection such as clones 6E10 and 4G8. In addition to detecting C-terminally processed fragments of APP, clone 3A1 shows preference for aggregated Aβ and demonstrates no cross-reactivity with APP. Clone M3.2 recognizes rodent Aβ, β-CTF, sAPP-α, and full-length APP. Clone M3.2 has negligible cross-reactivity with human isoforms of APP and Aβ. The antibodies provided in this kit are highly suitable for WB, IHC, and ELISA applications.Product Details
- Kit Contents
Specificity Format Clone Size Reactivity Isotype Anti-β-Amyloid, 1-16 Purified 6E10 25 μl Human Mouse IgG1 Anti-mouse/rat β-Amyloid, 1-16 Purified M3.2 25 μl Mouse, Rat Mouse IgG1, κ Anti-β-Amyloid, 1-40 Purified 11A50-B10 50 μl Human Mouse IgG1 Anti-β-Amyloid, 1-42 Purified 12F4 50 μl Human, Mouse, Rat Mouse IgG1 Anti-β-Amyloid, 1-15 Purified 3A1 25 μg Human Mouse IgG1, κ
* For detailed information about each specificity, please refer to the datasheets of the individual products.
- Please refer to individual product datasheets of the purified formats for details.
- All antibodies in this kit were purified by affinity chromatography.
- Storage & Handling
- Upon receipt, store undiluted at 2-8°C.
WB, IHC-P, IHC-F - Quality tested
- Recommended Usage
Each lot of antibodies in this kit is quality control tested by Western blotting. For Western blotting, the suggested uses of these reagents are as follows:
Anti-β-Amyloid, 1-16: 0.2 - 1.0 µg/ml
Anti-mouse/rat β-Amyloid, 1-16: 0.1 - 5.0 µg/ml
For immunohistochemistry on formalin-fixed paraffin-embedded tissue, the suggested use of this reagent is as follows:
Anti-β-Amyloid, 1-40: 5.0 - 10 µg/ml
Anti-β-Amyloid, 1-42: 0.2 - 5.0 µg/ml
For immunohistochemistry on frozen tissue, the suggested use of this reagent is as follows:
Anti-β-Amyloid, 1-15: 1.0 - 5.0 µg/ml
It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
For verified or reported applications for these antibodies, please see individual product datasheets.